Invitrogen™ EnzChek™ Lysozyme Assay Kit, 400 assays

Sensitive fluorescence-based assay for quantifying lysozyme activity in solution

The Invitrogen™ EnzChek™ Lysozyme Assay Kit offers a fast, simple, and highly sensitive method to measure lysozyme activity in solution using a fluorescence-based readout. Capable of detecting lysozyme concentrations as low as 20 U/mL, the kit is compatible with any fluorescence microplate reader or fluorometer set to standard fluorescein (FITC) excitation/emission settings.

The assay utilizes fluorescein-labeled Micrococcus lysodeikticus cell walls in which the fluorescence is quenched until the substrate is hydrolyzed by lysozyme. The enzyme cleaves the β-(1→4)-glucosidic linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine in the bacterial cell wall, relieving the quench and producing a dramatic fluorescence increase proportional to lysozyme activity.

Key Features

• High Sensitivity – Detects lysozyme activity as low as 20 U/mL
• Fluorescent Readout – Uses standard FITC excitation/emission (Ex ~494 nm / Em ~518 nm)
• Continuous Measurement – Enables real-time or endpoint analysis
• Easy to Use – Straightforward protocol compatible with standard lab equipment
• Quantitative – Linear correlation between fluorescence intensity and enzyme activity

Applications

• Lysozyme activity quantification in purified enzyme solutions
• Protein characterization and quality control
• Antimicrobial enzyme studies
• Enzyme kinetics and inhibitor screening
• Biotechnology, food safety, and pharmaceutical research

Specifications

The EnzChek™ Lysozyme Assay Kit delivers a sensitive, reproducible, and high-throughput-ready method for measuring lysozyme activity in solution. With its rapid setup and compatibility with standard lab instruments, it is an excellent choice for enzyme activity studies, kinetic assays, and inhibitor testing in both research and applied science settings.