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Cytiva HiTrap® Con A 4B Affinity chromatography column
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Specifications:
| Application | Protein Biology, Magnetic Affinity Separation |
| Storage Temperature | Room Temperature |
| Product Type | columns |
| Product Brand | Cytiva |
| Product Grade | Molecular Biology |
HiTrap™ Con A 4B columns are prepacked affinity chromatography columns containing Con A Sepharose™ 4B, designed for the selective purification of glycoproteins, glycolipids, and polysaccharides. The immobilized concanavalin A (Con A) specifically binds α-D-mannopyranosyl, α-D-glucopyranosyl, and related residues, making these columns ideal for lectin affinity purification workflows.
Compatible with manual methods or automated systems, HiTrap™ Con A 4B columns can be operated using a syringe, peristaltic pump, or ÄKTA chromatography system.
Key Features
- Prepacked and Ready-to-Use – Simplifies glycoprotein and carbohydrate purification
- High Binding Specificity – Con A binds α-D-mannopyranosyl and α-D-glucopyranosyl residues
- Multiple Formats – Available in 1 mL and 5 mL column sizes
- Flexible Operation – Compatible with manual and automated systems (e.g., ÄKTA design)
- Stable Resin – Con A coupled to Sepharose™ 4B for consistent performance
- Reusable under defined conditions – Stable within pH 4–9 and up to 5 bar pressure
Technical Specifications
| Property | 28-9520-85 | 28-9520-96 |
|---|---|---|
| Product Name | HiTrap™ Con A 4B | HiTrap™ Con A 4B |
| Catalog Number | 28-9520-85 | 28-9520-96 |
| Bed Volume | 1 mL per column (5-pack) | 5 mL per column (5-pack) |
| Column Hardware | Polypropylene (PP) | Polypropylene (PP) |
| Particle Size | 90 µm | 90 µm |
| Internal Diameter | 7.00 mm | 16.00 mm |
| Column Length | 25 mm | 25 mm |
| Maximum Pressure | 5 bar (0.5 MPa, 72 psi) | 5 bar (0.5 MPa, 72 psi) |
| Operating pH Range | 4–9 | 4–9 |
Applications
- Affinity Purification of Glycoproteins – Isolation of glycosylated proteins from serum, cell culture supernatants, or tissue extracts
- Lectin Binding Studies – Identification of glycan-binding properties in biological samples
- Glycoproteomic Research – Pre-enrichment of glycosylated targets for downstream mass spectrometry
- Carbohydrate-Binding Analysis – Characterization of glycan interactions and glycosylation patterns
- Vaccine and Biotherapeutic Development – Removal or concentration of glycosylated biopharmaceuticals
Binding Specificity
Con A is a tetrameric lectin extracted from Canavalia ensiformis (jack bean). It binds to:
- α-D-mannopyranosyl
- α-D-glucopyranosyl
- Other sterically related carbohydrate structures
Binding requires the presence of hydroxyl groups at C-3, C-4, and C-5 of the sugar ring.
Storage & Stability
- Shipping: Ambient temperature
- Storage: 2–8 °C, do not freeze
- Shelf Life: Refer to product label or datasheet
- Reusability: Columns may be reused under appropriate conditions; avoid exposure to harsh denaturants or strong reducing agents
Ordering Information
| Catalog # | Description | Pack Size |
|---|---|---|
| 28-9520-85 | HiTrap™ Con A 4B, 1 mL | 5 columns |
| 28-9520-96 | HiTrap™ Con A 4B, 5 mL | 5 columns |
HiTrap™ Con A 4B columns provide a reliable and efficient platform for lectin-based affinity purification, with strong specificity for glycosylated molecules. With prepacked convenience, compatibility across systems, and reproducible performance, they are ideal for use in biological, pharmaceutical, and glycoproteomic applications.
- Capacity: 5 mL 1 mL