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Specifications:
| Application | Protein Assays & Analysis | ||
| Storage Temperature | –80°C | ||
| Product Type | Protein Standards | Forms | Liquid |
| Product Brand | MyBioSource | ||
| Product Grade | Molecular Biology | ||
It has been known that the coronaviruses SARS-CoV-2 and SARS-CoV use human ACE2 as the entry receptor and human proteases as the entry activators. The virus surface spike (S) protein mediates SARS-CoV-2 entry into cells. To fulfill its function, SARS-CoV-2 spike binds to the human ACE2 (hACE2) receptor through its receptor-binding domain (RBD) and is proteolytically activated by human proteases.
MyBioScourse SARS-CoV-2 Pseudoviral Particles are replication-deficient MLV pseudotyped with the SARS-CoV-2 spike protein carrying the original D614 genotype (Genbank Accession # YP_009724390.1) of the original SARS-CoV-2 strain (the Wuhan-Hu-1 isolate. They also contain the ORF for firefly luciferase as a reporter. They establish a pseudovirus entry assay for SARS-CoV-2 as the spike protein mediated cell entry can be conveniently measured via the luciferase reporter activity. This pseudovirus assay isolates the SARS-CoV-2 viral entry from other steps of the viral infection cycle.
- Pack Size: 1 x 5 mL 5 x 1mL