Thermo Scientific™ Oxoid™ Brain Heart Infusion agar (Dehydrated)

The Thermo Scientific™ Oxoid™ Brain Heart Infusion Agar (Dehydrated) is a highly nutritious and versatile medium designed for the cultivation of fastidious microorganisms, including streptococci, Neisseria spp., and certain pathogenic fungi. It serves as a reliable general-purpose medium and can be supplemented with blood and antibiotics for specialized applications, such as the isolation of Histoplasma capsulatum and Coccidioides immitis.

Key Features

1. Highly Nutritious:
   • Supports the robust growth of fastidious bacteria and fungi.
2. Versatile Applications:
   • General-purpose medium for a wide variety of microorganisms.
   • Can be enriched for specific uses, such as fungal isolation.
3. Compatible with Supplements:
   • Addition of blood and antibiotics enables the cultivation of specialized organisms, including Histoplasma capsulatum.
4. Wide Compliance:
   • Meets industrial standards, including AFNOR, BSI, FDA, IDF, ISO, and USDA, for reliable use in diverse microbiological settings.
5. Cost-Effective:
   • High yield of 10.6L per 500g, suitable for routine laboratory use.

Applications

1. Clinical Microbiology

• Isolation and cultivation of Neisseria gonorrhoeae and streptococci.
• Diagnostic testing for fastidious pathogens.

2. Mycology

• With the addition of blood and antibiotics, it supports the isolation of pathogenic fungi, including:
  • Histoplasma capsulatum
  • Coccidioides immitis

3. General Microbiology

• Cultivation of a wide range of fastidious and non-fastidious microorganisms.

4. Research and Industrial Microbiology

• Suitable for research on fastidious organisms and for quality control in industrial microbiology.

Specifications

Preparation Instructions

1. Suspend: Suspend 47g of Brain Heart Infusion Agar in 1 liter of distilled water.
2. Mix and Heat: Heat with frequent agitation until fully dissolved.
3. Sterilize: Autoclave at 121°C for 15 minutes.
4. Enrich:
   • Add blood or antibiotics as needed for specific applications (e.g., fungal isolation).
5. Dispense: Pour into sterile Petri dishes or tubes and allow to solidify.

Advantages

1. Highly Nutritious: Supports a broad range of fastidious organisms.
2. Customizable: Can be enriched with blood or antibiotics for specialized applications.
3. Reliable and Versatile: Suitable for clinical, mycological, and general microbiology needs.
4. Cost-Effective: High yield for routine and large-scale testing.
5. Standard Compliant: Meets international microbiology standards for consistent results.

Diagnostic Features

Storage and Stability

• Storage: Store the dehydrated medium at 10–30°C in a dry environment.
• Shelf Life: Refer to the product label for expiration date.

Packaging

• Product: Brain Heart Infusion Agar (Dehydrated)
• Quantity: 500g
• Yield: 10.6L of prepared medium

References

1. Seth A. (1970). Brit. J. Vener. Dis., 46: 201–202.

The Thermo Scientific™ Oxoid™ Brain Heart Infusion Agar is a reliable and flexible medium for cultivating fastidious microorganisms. With its superior nutritional properties, it supports the growth of bacteria and fungi in clinical, research, and industrial microbiology settings. Its adaptability with supplements like blood and antibiotics makes it an indispensable tool for laboratories conducting routine and specialized microbial testing.