Thermo Scientific™ Oxoid™ Modified Semi-solid Rappaport Vassiliadis Agar (ISO) (Dehydrated)

The Thermo Scientific™ Oxoid™ Modified Semi-solid Rappaport Vassiliadis (MRSV) Agar (ISO) is a selective medium designed for the enrichment and isolation of Salmonella spp. from food, animal feces, and environmental samples. This medium utilizes motility enrichment, allowing Salmonella species to migrate through the selective medium ahead of competing organisms, forming distinct opaque halos. It complies with ISO 6579:2002+A1:2007 Annex D for Salmonella detection in animal production systems.

Key Features

1. Motility Enrichment:
   • Relies on the motility of Salmonella spp. to migrate ahead of competing Gram-negative organisms, improving specificity and sensitivity.
2. Highly Selective:
   • Suppresses Gram-positive flora using novobiocin, low pH, high magnesium chloride concentrations, and malachite green.
3. ISO Compliant:
   • Meets the requirements of ISO 6579:2002+A1:2007 Annex D for Salmonella detection in animal feces and production samples.
4. Wide Applicability:
   • Effective for testing food, environmental samples, and fecal material from poultry, pigs, cattle, and other animals.
5. Presumptive Identification:
   • Presumptive Salmonella colonies can be subcultured onto XLD Agar and other selective Salmonella agars for confirmation.

Applications

1. Food Microbiology

• Isolation of Salmonella spp. from raw and processed foods, including meat, dairy, and ready-to-eat products.

2. Veterinary Microbiology

• Detection of Salmonella in animal feces, feed, and dust samples from primary production systems.

3. Environmental Monitoring

• Monitoring Salmonella contamination in water, soil, and industrial settings.

4. Research Microbiology

• Studies on Salmonella motility, ecology, and antimicrobial resistance.

Specifications

Preparation Instructions

1. Suspend: Suspend 25g of MRSV Agar powder in 1 liter of distilled water.
2. Mix and Heat: Heat with frequent agitation until fully dissolved.
3. Sterilize: Autoclave at 121°C for 15 minutes.
4. Dispense: Pour the medium into sterile tubes or dishes and allow to solidify.

Advantages

1. Improved Recovery: Motility enrichment enhances the detection of Salmonella spp. by allowing them to outcompete other organisms.
2. Enhanced Selectivity: Suppresses competing flora through a combination of selective agents and medium properties.
3. ISO-Compliant: Suitable for regulated workflows requiring ISO standards for Salmonella testing.
4. Wide Utility: Applicable to diverse sample types, including food, animal, and environmental sources.
5. Simple and Reliable: Provides accurate presumptive identification for efficient workflows.

Quality Control

Packaging

• Product: Modified Semi-solid Rappaport Vassiliadis (MRSV) Agar (ISO)
• Catalog Number: CM1112B
• Quantity: 500g

The Thermo Scientific™ Oxoid™ Modified Semi-solid Rappaport Vassiliadis (MRSV) Agar (ISO) is an efficient and reliable medium for the selective enrichment and isolation of Salmonella spp. Its compliance with ISO standards, motility enrichment capability, and suitability for diverse sample types make it indispensable for microbiology laboratories focused on food safety, veterinary diagnostics, and environmental monitoring.