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Thermo Scientific™ Oxoid™ Brilliance™ UTI Clarity™ Agar (Dehydrated)
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Specifications:
| Application | Clinical microbiology | ||
| Storage Temperature | Room Temperature | ||
| Product Type | Culture Medium | Forms | Powder |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Microbiology grade | ||
The Thermo Scientific™ Brilliance™ UTI Clarity™ Agar is an advanced chromogenic medium designed for the isolation, enumeration, and presumptive identification of urinary tract infection (UTI) pathogens. It employs two highly specific chromogenic substrates and tryptophan to differentiate key organisms associated with UTIs, delivering clear, rapid, and reliable results.
Key Features
1. Chromogenic Differentiation
- Two Chromogenic Substrates:
- ß-galactosidase: Produces pink/pale pink colonies for E. coli and S. saprophyticus.
- ß-glucosidase: Produces blue/turquoise colonies for Enterococcus species.
- Dual enzyme activity (both ß-galactosidase and ß-glucosidase) in coliforms results in dark blue/purple colonies.
- Tryptophan Deaminase Activity:
- Produces a brown halo around colonies of Proteus spp., Morganella spp., and Providencia spp.
2. Improved Chromogens
- Incorporates advanced chromogens for:
- Enhanced color reactions.
- Easier differentiation of pathogen groups compared to traditional chromogenic media.
3. Rapid Testing
- Same-day results with the use of a rapid indole test (DMAC) for E. coli confirmation (Part No. MB1448A).
4. Reduced Confirmatory Tests
- High specificity reduces the need for additional confirmatory testing, streamlining workflows.
5. Clear and Easy-to-Read Results
- Diagnostic colony colors are distinct, making identification straightforward even in mixed cultures.
Applications
1. Urinary Tract Infection Diagnostics
- Isolate and identify common UTI pathogens like:
- E. coli
- Enterococcus spp.
- Proteus spp.
- Morganella spp.
- S. saprophyticus
2. Microbiology Laboratories
- Ideal for routine UTI pathogen identification in:
- Clinical microbiology labs.
- Hospital diagnostic centers.
- Public health laboratories.
3. Antimicrobial Resistance Monitoring
- Provides a platform for testing multidrug-resistant organisms (e.g., E. coli, Proteus spp.).
Advantages
1. Enhanced Specificity
- Minimizes false positives and reduces reliance on confirmatory tests.
2. Rapid Results
- Enables same-day identification of E. coli using a rapid indole test.
3. Simplified Workflow
- Combines isolation, differentiation, and presumptive identification in a single medium.
4. High Diagnostic Value
- Distinct colony colors ensure easy identification of mixed cultures.
How It Works
- Inoculation:
- Spread the urine sample onto the agar plate.
- Incubation:
- Incubate at 35–37°C for 18–24 hours under aerobic conditions.
- Interpretation:
- Observe colony colors:
- E. coli, S. saprophyticus: Pink/pale pink.
- Enterococcus spp.: Blue/turquoise.
- Coliforms: Dark blue/purple.
- Proteus spp., Morganella spp., Providencia spp.: Brown halo.
- Other organisms: Natural pigmentation.
- Observe colony colors:
- Confirmatory Testing:
- Perform a rapid indole test (DMAC) for same-day confirmation of E. coli.
Specifications
| Parameter | Details |
|---|---|
| Medium Type | Chromogenic agar |
| Target Organisms | UTI pathogens |
| Incubation Time | 18–24 hours |
| Incubation Temperature | 35–37°C |
| Storage Conditions | 2–8°C |
| Applications | Routine UTI diagnostics |
Why Choose Brilliance™ UTI Clarity™ Agar?
- Accuracy: Superior specificity and chromogenic reactions.
- Efficiency: Reduces confirmatory testing, saving time and resources.
- Speed: Enables rapid same-day identification of E. coli.
- Ease of Use: Clear colony colors ensure quick and accurate results.
The Brilliance™ UTI Clarity™ Agar is an ideal medium for laboratories seeking a reliable and efficient solution for UTI pathogen isolation and differentiation.
Comparison: CHROMagar™ UTI vs. Brilliance™ UTI Clarity™ Agar
| Feature | CHROMagar™ UTI | Brilliance™ UTI Clarity™ Agar |
|---|---|---|
| Pathogen Differentiation | Differentiates E. coli, Proteus spp., Klebsiella spp., Enterococcus spp., S. saprophyticus, and other coliforms. | Differentiates E. coli, Proteus spp., Morganella spp., Providencia spp., Enterococcus spp., S. saprophyticus, and coliforms. |
| Chromogenic Substrates | Uses chromogens for enzyme reactions targeting ß-galactosidase and ß-glucosidase activities. | Utilizes enhanced chromogens for more specific enzymatic reactions and tryptophan for detecting tryptophan deaminase activity. |
| Swarming Control | Inhibits Proteus spp. swarming. | Prevents Proteus spp. swarming through specific inhibitory mechanisms. |
| Colony Appearance | Produces distinct colors for various pathogens: E. coli (pink to dark rose), coliforms (blue to violet), Proteus spp. (brown halo). | Provides enhanced color differentiation: E. coli (pink/pale pink), coliforms (dark blue/purple), Proteus spp. (brown halo), Enterococcus (blue/turquoise). |
| Specificity | High, suitable for common UTI pathogens. | Very high, with improved differentiation of Morganella and Providencia. |
| Turnaround Time | Results within 18–24 hours. | Same-day results possible for E. coli using a rapid indole test (DMAC). |
| Ease of Use | Easy-to-read colonies with distinct colors. | Clearer colony differentiation with more diagnostic colors for mixed cultures. |
| Confirmatory Testing | Requires confirmatory tests for some organisms. | Reduces confirmatory tests due to high specificity. |
| Compatibility with Automation | Compatible with automated streaking systems. | Compatible with manual and automated processes. |
Applications
| Application | CHROMagar™ UTI | Brilliance™ UTI Clarity™ Agar |
|---|---|---|
| Routine UTI Diagnostics | Highly effective for identifying common UTI pathogens. | Provides broader and clearer differentiation, especially in mixed cultures. |
| Multidrug-Resistant Pathogens | Effective for identifying antibiotic-resistant strains like E. coli. | Enhanced ability to detect Morganella spp. and Providencia spp., commonly associated with resistance. |
| Mixed Cultures | Can differentiate organisms but may require additional confirmatory tests. | Superior differentiation with reduced need for confirmatory testing. |
| Rapid Testing | Standard incubation times (18–24 hours). | Faster results for E. coli when using DMAC indole test. |
| Cost-Effectiveness | Cost-effective and widely used. | Higher specificity and reduced confirmatory tests may offset higher initial cost. |
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