Thermo Scientific™ Oxoid™ Campylobacter Karmali Agar Base (Dehydrated)

The Thermo Scientific™ Oxoid™ Campylobacter Karmali Agar Base (Dehydrated) is a selective medium designed for the isolation of Campylobacter jejuni and Campylobacter coli from clinical specimens. Based on the formulation by Karmali et al., this medium is used with Modified Karmali Selective Supplement (SR0205) or Campylobacter Selective Supplement (Karmali) (SR0167). The formulation provides robust selectivity and visibility, making it ideal for identifying Campylobacter spp. in diagnostic microbiology workflows.

Key Features

1. Selective Formulation:
   • Includes vancomycin and amphotericin B to suppress Gram-positive organisms and fungi, enhancing the recovery of Campylobacter spp.
2. Blood-Free Alternative:
   • Incorporates sodium pyruvate in the supplement, eliminating the need for blood in the medium.
3. Clear Colony Morphology:
   • Campylobacter jejuni produces grey, moist, flat spreading colonies after 42-hour incubation at 42°C, simplifying identification.
4. Wide Applicability:
   • Suitable for testing clinical, food, and environmental samples.
5. Validated and Reliable:
   • Recommended for Campylobacter isolation by microbiology standards.

Applications

1. Clinical Microbiology

• Isolation and identification of Campylobacter spp. from human fecal samples to diagnose enteritis and other gastrointestinal illnesses.

2. Food Microbiology

• Detection of Campylobacter in raw and processed foods, including:
  • Meat and poultry
  • Dairy products
  • Seafood

3. Environmental Microbiology

• Monitoring Campylobacter contamination in water, soil, and food production environments.

Specifications

Supplement Compatibility

Preparation Instructions

1. Suspend: Suspend 42g of Campylobacter Karmali Agar Base in 1 liter of distilled water.
2. Mix and Heat: Heat with frequent agitation to dissolve completely.
3. Sterilize: Autoclave at 121°C for 15 minutes.
4. Supplement: After cooling to 45–50°C, aseptically add the appropriate Karmali Selective Supplement.
5. Dispense: Pour the prepared medium into sterile Petri dishes and allow it to solidify.

Advantages

1. Targeted Selectivity: Effectively suppresses non-target organisms, ensuring accurate recovery of Campylobacter spp.
2. Blood-Free Design: Simplifies preparation while maintaining robust performance.
3. Clear Differentiation: Colonial morphology supports quick and reliable identification of Campylobacter jejuni.
4. Wide Application: Suitable for clinical, food, and environmental microbiology workflows.
5. Validated Formulation: Trusted for use in diagnostic and research laboratories.

Storage and Stability

• Storage: Store the dehydrated medium at 10–30°C in a dry environment.
• Shelf Life: Refer to the product label for expiration date.

Packaging

• Product: Campylobacter Karmali Agar Base (Dehydrated)
• Quantity: 500g

The Thermo Scientific™ Oxoid™ Campylobacter Karmali Agar Base is a reliable, selective medium for the isolation and identification of Campylobacter jejuni and Campylobacter coli. Its selective agents and robust formulation ensure accurate results, making it a valuable tool for microbiology laboratories focused on clinical diagnostics, food safety, and environmental monitoring.