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Thermo Scientific™ Oxoid™ Fraser Broth (Dehydrated)
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Specifications:
| Application | Microbiology | ||
| Storage Temperature | Ambient | ||
| Product Type | Culture Medium | Forms | Powder |
| Product Brand | Thermo Fisher Scientific™, Oxoid | ||
| Product Grade | Microbiology grade | ||
The Thermo Scientific™ Oxoid™ Fraser Broth Base (Dehydrated) is a selective enrichment medium specifically designed for the detection, isolation, and enumeration of Listeria spp., including Listeria monocytogenes, from food and environmental samples. Conforming to ISO 11290-1:2017, this medium is essential for food safety and quality assurance laboratories. The selective nature of Fraser Broth is enhanced by its ability to provide presumptive evidence of Listeria through blackening of the medium.
Key Features
- Selective Enrichment:
- Optimized for the recovery of Listeria spp. from food and environmental samples, even in the presence of competing flora.
- ISO Compliance:
- Fully compliant with ISO 11290-1:2017, ensuring adherence to international food safety standards.
- Presumptive Identification:
- Blackening of the medium serves as an early indicator of Listeria spp. presence, streamlining workflows.
- Wide Application:
- Suitable for testing raw and processed foods, environmental samples, and production facilities.
- Reliable and Reproducible:
- Delivers consistent results for Listeria detection in routine and high-throughput testing workflows.
Applications
1. Food Microbiology
- Detection and recovery of Listeria spp. from raw and processed foods, including:
- Dairy products
- Ready-to-eat foods
- Meat and poultry
- Seafood
2. Environmental Monitoring
- Isolation of Listeria from environmental swabs and production facility surfaces.
3. Regulatory and Compliance Testing
- Meets ISO, AFNOR, IDF, and NMKL standards for Listeria detection in food safety workflows.
Specifications
| Attribute | Details |
|---|---|
| Product Type | Dehydrated Culture Media |
| Form | Powder |
| Description | Fraser Broth |
| Selective Agent | Selective for Listeria spp. |
| Quantity | 500g |
| Yield | 8.7L of prepared medium |
| Certifications | AFNOR, IDF, ISO, NMKL |
Preparation Instructions
- Suspend: Suspend 57.5g of Fraser Broth Base powder in 1 liter of distilled water.
- Mix and Heat: Heat with frequent agitation to dissolve completely.
- Sterilize: Autoclave at 121°C for 15 minutes.
- Cool: Allow to cool to appropriate inoculation temperature.
Advantages
- High Selectivity: Recovers Listeria spp. even in complex samples with competing flora.
- Early Identification: Blackening of the medium provides presumptive evidence of Listeria presence.
- Compliance Ready: Fully meets ISO and other regulatory standards.
- Broad Utility: Applicable for food, environmental, and compliance testing workflows.
- Cost-Effective: High yield of 8.7L per 500g ensures economical use in routine testing.
Diagnostic Features
| Organism | Reaction |
|---|---|
| Listeria spp. | Blackening of the medium (positive) |
| Non-Listeria organisms | No blackening of the medium |
Storage and Stability
- Storage: Store the dehydrated medium at 10–30°C in a dry environment.
- Shelf Life: Refer to product label for expiration date.
Packaging
- Product: Fraser Broth Base (Dehydrated)
- Catalog Number: CM0895B
- Quantity: 500g
- Yield: 8.7L of prepared medium
The Thermo Scientific™ Oxoid™ Fraser Broth Base (Dehydrated) is a trusted medium for the selective enrichment and isolation of Listeria spp. Its ISO compliance, ease of use, and reliable performance make it an essential tool for microbiology laboratories focused on food safety, environmental monitoring, and regulatory compliance. The presumptive blackening reaction provides an early indication of contamination, enhancing efficiency in detection workflows.