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Thermo Scientific™ Oxoid™ Alternative Thioglycolate Broth
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Specifications:
| Application | Microbiology | ||
| Storage Temperature | Room Temperature | ||
| Product Type | Culture Medium | Forms | Powder |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Microbiology grade | Formula | For 17.2L medium |
This broth serves as an alternative to Thioglycollate Medium USP CM0173, specifically designed for sterility testing of turbid or viscous biological products. The formulation excludes agar and resazurin, making it essential for freshly prepared or recently boiled medium usage.
Key Features
- Application:
- Used for sterility testing of turbid/viscous samples where regular thioglycollate media are unsuitable due to viscosity.
- Suitable for inactivating mercurial preservatives (up to 0.03% w/v) in inocula.
- Advantages:
- Lacks agar and resazurin, ensuring better adaptability for challenging biological products.
- Ideal for samples requiring freshly prepared or minimally oxidized media.
- Compatibility:
- Recommended for use with turbid or viscous inocula.
- Designed to meet standards outlined in N.I.H. Memorandum and USP XXI.
Typical Formula (gm/litre)
- L-cystine: 0.5
- Sodium chloride: 2.5
- Glucose: 5.5
- Yeast extract: 5.0
- Pancreatic digest of casein: 15.0
- Sodium thioglycollate: 0.5
- pH: 7.1 ± 0.2 @ 25°C
Preparation Instructions
- Dissolution: Suspend 29g of the medium in 1 litre of distilled water and bring to a boil until fully dissolved.
- Sterilization: Distribute the medium into tubes or bottles and autoclave at 121°C for 15 minutes.
- Usage: Prepare freshly or boil and cool the medium within four hours before use.
Storage & Shelf Life
- Dehydrated Medium: Store at 10–30°C.
- Prepared Medium: Not recommended for storage; can be held at 20–30°C in the dark for short periods.
- Avoid reheating the medium multiple times to prevent the formation of toxic oxygen radicals.
Appearance
- Dehydrated Medium: Straw-colored, free-flowing powder.
- Prepared Medium: Straw-colored solution.
Quality Control
| Control Organisms | Expected Results |
|---|---|
| Candida albicans ATCC® 10231 | Flocculent growth |
| Clostridium sporogenes ATCC® 19404 | Turbid growth and/or colonies |
| Peptostreptococcus anaerobius ATCC® 27337 | Turbid growth and/or colonies |
| Negative Control | No change (uninoculated medium) |
Precautions
- Use the medium freshly prepared or within four hours of preparation.
- Avoid reheating the medium multiple times to prevent oxidative damage.
- Ensure appropriate storage conditions to maintain efficacy.
Thermo Scientific™ Oxoid™ USP Alternative Thioglycolate Broth (CM0391B) vs. Thioglycollate Medium USP (CM0173B)
| Feature | CM0391B: USP Alternative Thioglycollate Broth | CM0173B: Thioglycollate Medium USP |
|---|---|---|
| Intended Use | Sterility testing for turbid/viscous products | General sterility testing |
| Formula Components | - L-cystine | - L-cystine |
| - Sodium chloride | - Sodium chloride | |
| - Glucose | - Glucose | |
| - Yeast extract | - Yeast extract | |
| - Pancreatic digest of casein | - Pancreatic digest of casein | |
| - Sodium thioglycollate | - Sodium thioglycollate | |
| No agar or resazurin | Includes agar and resazurin | |
| pH | 7.1 ± 0.2 at 25°C | 7.1 ± 0.2 at 25°C |
| Recommended Preparation | Dissolve 29g/L, sterilize at 121°C for 15 minutes | Dissolve 29g/L, sterilize at 121°C for 15 minutes |
| Preparation Notes | Must be freshly prepared or boiled and used within 4 hours | Can be stored for longer periods |
| Consistency | Liquid medium (no agar) | Slightly viscous due to agar |
| Key Applications | - Testing viscous/turbid products | - General sterility testing |
| - Culturing anaerobic and facultative microorganisms | - Culturing anaerobic and facultative microorganisms | |
| - Detecting flocculent or turbid growth | - Detecting growth through turbidity or visual indicators | |
| Key Features | - Non-viscous, suited for turbid inocula | - Includes resazurin as an oxidation-reduction indicator |
| - Requires fresh preparation or reheating immediately before use | - Agar component improves stability and facilitates anaerobic growth | |
| Quality Control | - Candida albicans ATCC® 10231: Flocculent growth | - Candida albicans ATCC® 10231: Flocculent growth |
| - Clostridium sporogenes ATCC® 19404: Turbid growth/colonies | - Clostridium sporogenes ATCC® 19404: Turbid growth/colonies | |
| - Peptostreptococcus anaerobius ATCC® 27337: Turbid growth/colonies | - Peptostreptococcus anaerobius ATCC® 27337: Turbid growth/colonies | |
| - No change in uninoculated medium | - No change in uninoculated medium | |
| Storage Conditions | Dehydrated: 10–30°C | Dehydrated: 10–30°C |
| Prepared: Use freshly or within 4 hours | Prepared: Store at 20–30°C, away from light | |
| Appearance | Dehydrated: Straw-colored, free-flowing powder | Dehydrated: Straw-colored, free-flowing powder |
| Prepared: Straw-colored solution | Prepared: Slightly viscous, straw-colored solution | |
| Key Advantage | Optimized for sterility testing in viscous or turbid biological products | General-purpose sterility medium with resazurin for easy visualization |
| Limitation | Requires immediate use after preparation | Less effective for viscous or turbid samples |
This table highlights the differences and similarities between the two Thioglycollate media types, helping users select the appropriate medium based on their specific application needs.
References
- N.I.H. Memorandum (1955): Culture media for sterility tests (4th Revision).
- USP XXI (1985): Sterility Tests.
This broth provides a reliable solution for sterility testing of turbid and viscous products, adhering to strict quality and performance standards.
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