Thermo Scientific™ Oxoid™ Gc Agar Base (Dehydrated)

The Thermo Scientific™ Oxoid™ GC Agar Base (Dehydrated) is a specialized basal medium designed for the isolation of Neisseria species, including Neisseria gonorrhoeae and other fastidious organisms. This medium contains Special Peptone LP0072, a combination of meat and plant enzymatic digests, which provides essential nutrients for bacterial growth. The inclusion of starch absorbs toxic metabolites produced by Neisseria, while phosphate buffers stabilize the pH, ensuring optimal survival and growth of these organisms.

Key Features

1. Nutrient-Rich Composition:
   • Includes Special Peptone LP0072 for enhanced support of fastidious Neisseria spp.
2. Starch for Metabolite Absorption:
   • Absorbs toxic metabolites produced by Neisseria, promoting better growth and viability.
3. pH Stability:
   • Phosphate buffers prevent pH changes due to amine production, ensuring consistent growth conditions.
4. Versatile Basal Medium:
   • Used in the preparation of Chocolate Agar, Thayer-Martin Medium, Modified Thayer-Martin Medium, Martin-Lewis Agar, and Transgrow Agar.
5. Diagnostic Utility:
   • Ideal for clinical samples in workflows aimed at detecting and identifying bacterial infections.

Applications

1. Clinical Microbiology

• Isolation and identification of Neisseria gonorrhoeae from clinical samples.
• Support for the growth of other fastidious organisms in diagnostic workflows.

2. Research Microbiology

• Cultivation of Neisseria spp. for research purposes, including antibiotic susceptibility studies.

3. Preparation of Specialized Media

• Basal medium for creating enriched and selective media such as:
  • Chocolate Agar
  • Thayer-Martin Medium
  • Modified Thayer-Martin Medium
  • Martin-Lewis Agar
  • Transgrow Agar

Specifications

Preparation Instructions

1. Suspend: Suspend 36g of GC Agar Base in 1 liter of distilled water.
2. Mix and Heat: Heat with frequent agitation to dissolve completely.
3. Sterilize: Autoclave at 121°C for 15 minutes.
4. Supplement if Required: Add appropriate supplements or enrichments depending on the intended application (e.g., blood for Chocolate Agar, antibiotics for Thayer-Martin Medium).
5. Dispense: Pour into sterile Petri dishes or tubes and allow to solidify.

Advantages

1. High Nutritional Value: Supports the growth of fastidious organisms such as Neisseria spp.
2. Reliable Performance: Maintains pH stability and absorbs toxic metabolites for consistent results.
3. Versatile Applications: Can be used as a basal medium for multiple specialized media.
4. Cost-Effective: High yield of 13.9L per 500g, suitable for routine laboratory use.
5. Broad Utility: Applicable in clinical, diagnostic, and research workflows.

Storage and Stability

• Storage: Store the dehydrated medium at 10–30°C in a dry environment.
• Shelf Life: Refer to the product label for expiration date.

Packaging

• Product: GC Agar Base (Dehydrated)
• Catalog Number: CM0367B
• Quantity: 500g

The Thermo Scientific™ Oxoid™ GC Agar Base (Dehydrated) is a reliable and versatile basal medium for isolating Neisseria spp. and other fastidious organisms. Its nutrient-rich formulation, pH stability, and ability to support multiple specialized media make it an indispensable tool for clinical diagnostics and research laboratories.