Thermo Scientific™ Oxoid™ WL Nutrient Agar

Thermo Scientific™ WL Nutrient Agar is a reliable medium for isolating and enumerating microbial flora in brewing and fermentation applications. It is specifically formulated to assess microbial contamination in brewing and fermentation processes. Based on the medium designed by Green and Gray, WL Nutrient Agar is an essential tool for microbiological flora determination in these industrial processes.

This medium provides accurate microbial counts in the fermentation process, offering optimal performance at a pH of 5.5, which is ideal for counting microbial populations during brewing and fermentation. The medium can also be adjusted to pH 6.5, which is better suited for counting bakers' and distillers' yeasts.

Additionally, Cycloheximide (SR0222) can be added to the medium at a final concentration of 4 mg/L, which suppresses yeast growth and makes the medium selective for bacterial contaminants in yeast cultures.

Key Features:

1. Microbial Flora Analysis:
   • Specifically designed to assess the microbial flora in brewing and fermentation processes, ensuring quality control and monitoring.
2. Dual pH Adjustment:
   • pH 5.5 is optimal for reliable microbial counts during brewing and fermentation, while pH 6.5 is ideal for bakers' and distillers' yeast analysis.
3. Cycloheximide Additive:
   • Adding Cycloheximide at 4 mg/L suppresses yeast growth, enabling selective isolation of bacterial contaminants in yeast cultures.
4. Precise Incubation Conditions:
   • Incubation at 25°C is used for brewing materials, and 30°C is ideal for bakers' yeasts, ensuring accurate and efficient microbial growth for both industries.
5. Reliable and Consistent Results:
   • Based on the original Green and Gray formula, ensuring reliable microbial enumeration for quality control in brewing and fermentation.
6. Laboratory Use Only:
   • This product is for research and industrial applications, not intended for clinical or diagnostic use.

Specifications:

Applications:

1. Microbial Flora Analysis in Brewing:
   • Used for determining microbial contamination in brewing materials, allowing for accurate control of microbial growth during beer production.
2. Fermentation Process Monitoring:
   • Widely used for identifying and enumerating microbes in fermentation processes, critical for ensuring fermentation quality and productivity.
3. Yeast Analysis:
   • pH adjustments (6.5) make it suitable for bakers' and distillers' yeasts analysis, helping ensure optimal fermentation efficiency.
4. Bacterial Contamination Detection in Yeast Cultures:
   • With Cycloheximide addition, the medium is selective for bacterial contaminants, useful in maintaining pure yeast cultures for fermentation.

Related Products:

• Thermo Scientific™ Nutrient Agar for Microbial Analysis
• Thermo Scientific™ Cycloheximide Solution
• Thermo Scientific™ Yeast Extract for Culturing

General Reference:

• Green, S. R. and Gray, P. P. (1950) Wallerstein Lab. Comm. 13. 357.

The Thermo Scientific™ WL Nutrient Agar is an essential medium for monitoring microbial flora in brewing and fermentation applications. Its ability to isolate and enumerate microbes effectively, combined with the optional use of Cycloheximide for selective bacterial contamination analysis, makes it a vital tool in quality control for both brewers and fermentation industry professionals.