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Thermo Scientific™ Oxoid™ Blood Agar Base No. 2 (Dehydrated)
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Specifications:
| Application | Microbiology | ||
| Storage Temperature | Ambient | ||
| Product Type | Culture Medium | Forms | Powder |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Microbiology grade | ||
The Thermo Scientific™ Oxoid™ Blood Agar Base No. 2 (Dehydrated) is an improved, highly nutritious basal medium designed to support the growth and isolation of fastidious microorganisms and pathogens. This versatile medium promotes superior growth, early colonial development, and enhanced pigment formation in chromogenic bacteria. When enriched with blood, it enables the determination of hemolytic reactions, a key diagnostic feature for many organisms, including streptococci and pneumococci.
Key Features
- Improved Formulation:
- Superior to standard Blood Agar Base, offering enhanced growth-promoting properties and pigment formation.
- Supports Fastidious Microorganisms:
- Promotes luxuriant growth of streptococci, pneumococci, and other delicate organisms.
- Diagnostic Utility:
- With the addition of blood, facilitates clear identification of hemolytic reaction patterns (alpha, beta, gamma hemolysis).
- Versatile Applications:
- Serves as a basal medium for enriched blood agar or as a general-purpose medium for maintaining stock cultures.
- Cost-Effective:
- High yield of 12.5L per 500g, suitable for routine laboratory use.
Applications
1. Clinical Microbiology
- Isolation and identification of pathogens from clinical samples, including:
- Throat swabs
- CSF samples
- Pus, wound, and abscess swabs
- Respiratory and sputum cultures
2. Food and Environmental Microbiology
- Cultivation of fastidious pathogens from food or environmental samples.
- Monitoring contamination in food production facilities and equipment.
3. Research and Industrial Microbiology
- Research on chromogenic and fastidious organisms.
- Investigation of hemolytic reaction patterns for diagnostic purposes.
Specifications
| Attribute | Details |
|---|---|
| Product Type | Agar |
| Form | Powder |
| Description | Blood Agar Base No. 2 |
| Quantity | 500g |
| Yield | 12.5L of prepared medium |
| Intended Use | General-purpose medium for microbial isolation |
| Unit Size | Each |
Preparation Instructions
- Suspend: Suspend 40g of Blood Agar Base No. 2 in 1 liter of distilled water.
- Mix and Heat: Heat with frequent agitation until completely dissolved.
- Sterilize: Autoclave at 121°C for 15 minutes.
- Enrich:
- Add 5–10% sterile defibrinated blood for hemolysis determination.
- Add specific supplements as required for enhanced growth or diagnostic studies.
- Dispense: Pour into sterile Petri dishes and allow to solidify.
Advantages
- Enhanced Growth: Supports the early colonial development of fastidious organisms.
- Diagnostic Clarity: Clear differentiation of hemolytic reactions when blood is added.
- Versatility: Can be used with or without supplements for a range of applications.
- Reliable and Cost-Effective: Consistent performance across clinical, food, and research microbiology.
- Customizable: Suitable for enriching with various supplements to meet specific laboratory needs.
Diagnostic Features
| Additive | Application |
|---|---|
| Defibrinated Blood | Detection of hemolysis patterns (alpha, beta, gamma). |
| Supplements | Enhanced growth of fastidious organisms. |
Storage and Stability
- Storage: Store the dehydrated medium at 10–30°C in a dry environment.
- Shelf Life: Refer to the product label for expiration date.
Packaging
- Product: Blood Agar Base No. 2 (Dehydrated)
- Quantity: 500g
- Yield: 12.5L of prepared medium
The Thermo Scientific™ Oxoid™ Blood Agar Base No. 2 (Dehydrated) is a robust, versatile medium designed to meet the needs of clinical, food, and research microbiology laboratories. With its improved formulation and ability to support fastidious organisms and hemolytic testing, this medium is indispensable for routine and specialized microbiological testing.