Thermo Scientific™ Oxoid™ Tryptone Glucose Extract Agar (Dehydrated)

Thermo Scientific™ Oxoid™ Tryptone Glucose Extract Agar (TGE Agar) is a nutrient-rich medium specifically designed for microbial plate counts in water, dairy products, and the detection of thermophilic bacteria. It adheres to the standards recommended by the American Public Health Association (APHA).

Key Features:

1. Multipurpose Application:
   • Ideal for plate counts of water and dairy products.
   • Suitable for detecting thermophilic organisms in dairy samples.
2. Supplementation with Milk:
   • When sample dilutions exceed 1:10, sterile Skim Milk Powder solution can be added for enhanced performance.
3. Standardized Formula:
   • Formulated to meet performance requirements specified in APHA guidelines.
4. Minimizes Overheating Risk:
   • Overheating during sterilization is avoided to preserve medium integrity.

Typical Formula (per liter):

Preparation Instructions:

1. Reconstitution:
   • Suspend 24 g of the dehydrated medium in 1 liter of distilled water.
   • Heat to boiling with frequent stirring until dissolved completely.
2. Sterilization:
   • Autoclave at 121°C for 15 minutes.
3. Supplementation (if required):
   • Add 10 mL of sterile 10% Skim Milk Powder solution per liter if the dilution is greater than 1:10.
4. Mixing:
   • Mix thoroughly before pouring into sterile Petri dishes.

Applications:

1. Plate Count Analysis:
   • Enumeration of microorganisms in water and dairy products.
2. Detection of Thermophiles:
   • Ideal for identifying thermophilic bacteria in dairy processes.
3. Quality Control:
   • For monitoring microbial content in pasteurized and unpasteurized milk.

Specifications:

Quality Control:

Storage and Shelf Life:

• Dehydrated Medium:
  • Store at 10–30°C in a tightly sealed container.
  • Shelf life: Up to 2 years (check packaging for expiry date).
• Prepared Medium:
  • Store at 2–8°C.
  • Use within the recommended time frame for optimal performance.

Appearance:

Advantages:

Precautions:

• Avoid overheating the medium during preparation to prevent degradation.
• Handle and prepare the medium under aseptic conditions to avoid contamination.

References:

1. American Public Health Association (1980). Standard Methods for the Examination of Water and Wastewater. 15th Edition, APHA, Washington DC.
2. American Public Health Association (1972). Standard Methods for the Examination of Dairy Products. 13th Edition, APHA, Washington DC.

For reliable plate counts and thermophile detection, Thermo Scientific™ Oxoid™ Tryptone Glucose Extract Agar offers proven performance and versatility in water and dairy microbiology testing.