Invitrogen™ One Shot™ OmniMAX™ 2 T1R Chemically Competent E. coli, 21 x 50 µL/tube

Invitrogen One Shot™ OmniMAX™ 2 T1R Chemically Competent E. coli is a highly efficient strain designed for a wide range of molecular cloning applications, including Gateway cloning and TOPO PCR cloning. These cells combine the highest transformation efficiency with genetic modifications for stability, versatility, and reliable results.

Key Features:

1. Superior Transformation Efficiency:
   • Achieves >5 × 10⁹ cfu/μg pUC19 DNA, the highest transformation efficiency among chemically competent E. coli strains.
2. Enhanced Versatility:
   • Compatible with various cloning applications, including Gateway cloning and TOPO PCR cloning.
   • lacZΔM15 genotype enables blue-white screening using X-Gal or Bluo-Gal.
3. Genetic Modifications for Stability:
   • recA1 Mutation: Reduces nonspecific recombination in cloned DNA.
   • endA1 Mutation: Eliminates nonspecific digestion by Endonuclease I, improving plasmid quality.
   • tonA Mutation: Confers resistance to T1 and T5 phage infection, ensuring clone security.
   • Δ(ccdAB): Allows negative selection of ccdB gene-containing vectors in Gateway cloning.
   • mcrA, mcrBC, mrr, and hsdRMS Mutations: Enable efficient cloning of methylated DNA and enhance library representation.
4. Convenient Single-Tube Format:
   • The One Shot™ format allows all steps of the transformation process, including plating, to be performed in the same tube, minimizing contamination risk and saving time.
5. F´ Episome:
   • Contains lacIq for inducible expression from trc, tac, and lac promoters using IPTG.
   • Stable episome for plasmids with f1-like origins of replication.

Applications:

• High-Efficiency Cloning:
  • Ideal for cloning plasmids, PCR products, and cDNA.
• Blue/White Screening:
  • Facilitates visual identification of successful transformants.
• Gateway Cloning:
  • Optimized for negative selection of vectors with the ccdB gene.
• TOPO PCR Cloning:
  • Designed for seamless PCR product cloning.
• Library Construction:
  • Compatible with constructing genomic and cDNA libraries using plasmid vectors.

Specifications:

Genotype:

• F´ [proAB+ lacIq lacZΔM15 Tn10(TetR) Δ(ccdAB)] mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 Δ(lacZYA-argF)U169 endA1 recA1 supE44 thi-1 gyrA96 (NalR) relA1 tonA panD

Kit Contents:

Advantages:

1. High Transformation Efficiency: Facilitates successful cloning of even low-abundance DNA.
2. Improved DNA Quality: EndA1 mutation eliminates nonspecific DNA digestion.
3. Versatile Application: Supports a wide range of molecular cloning techniques.
4. Secure Cloning: Phage resistance and plasmid stability enhance experimental reliability.

Handling and Storage:

• Store Competent Cells: -80°C.
• Store pUC19 DNA: -20°C.
• Store S.O.C. Medium: 4°C or at room temperature.