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Specifications:
| Application | Flow Cytometry | ||
| Storage Temperature | Ambient | ||
| Product Type | Flow Cytometry Reagents | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
Invitrogen™ CountBright™ Plus Ready Tubes are a pre-calibrated, lyophilized bead-based solution for absolute cell counting in flow cytometry—offering the same reliable performance as CountBright™ Plus Absolute Counting Beads, now in a convenient, ready-to-use tube format. With no pipetting or reagent preparation required, these tubes improve workflow accuracy and reproducibility while minimizing handling errors.
Each tube contains a fixed quantity of ~4 µm fluorescent beads that are excitable across UV to IR laser ranges and fluoresce brightly across 385–860 nm, ensuring compatibility with nearly all flow cytometers. CountBright™ Plus Ready Tubes are especially well-suited for Lyse/No-Wash and No-Lyse/No-Wash protocols, but can be used in any flow cytometric assay requiring absolute cell enumeration.
Key Features and Benefits
- Preloaded, Ready-to-Use Tubes: Eliminates pipetting errors and saves time
- Room Temperature Stability: Convenient 12-month benchtop storage—no refrigeration needed
- Uniform Beads (~4 µm): Matches typical cell size, allowing for accurate comparison and gating
- High Singlet Content (>95%): Reduces doublet formation, enhancing quantification accuracy
- Broad Fluorescence Range (385–860 nm): Compatible with most flow cytometer lasers
- Optimized for Modern Workflows: Ideal for simplified sample prep and automated protocols
Applications
- Absolute cell counting in flow cytometry
- Standardization of cell concentration in assays
- Quantification of cell populations in whole blood, PBMCs, or cultured cells
- Quality control in viability, proliferation, and immune profiling assays
- Compatible with Lyse/No-Wash and No-Lyse/No-Wash sample prep protocols
- Instrument calibration and data normalization across experiments
Technical Specifications
| Property | Specification |
|---|---|
| Product Type | Cell Counting Bead |
| Format | Tubes (pre-loaded lyophilized beads) |
| Bead Diameter | ~4 µm |
| Fluorescence Emission | 385–860 nm |
| Number of Tests | 50 Tests |
| Bead Form | Lyophilized, pre-calibrated |
| Storage Conditions | Room temperature (up to 12 months shelf life) |
| Shipping Condition | Room temperature |
| Application | Flow Cytometry |
| Product Line | CountBright™ |
| Unit Size | Each |
Invitrogen™ CountBright™ Plus Ready Tubes simplify and standardize absolute cell counting in flow cytometry. With pre-measured, lyophilized beads in each tube, they eliminate pipetting variability and reduce prep time, enabling consistent and reproducible results. Their broad fluorescence range, high singlet purity, and room temperature storage make them a practical and reliable solution for researchers performing quantitative cytometry in immunology, hematology, oncology, and general cell biology.