Invitrogen™ Human PD-L1 ELISA Kit

The Invitrogen™ Human PD-L1 ELISA Kit is a highly sensitive tool designed for the quantitative detection of Human Programmed Death Ligand 1 (PD-L1/CD274) in various sample types, including serum, plasma, cell lysates, and supernatants. This sandwich ELISA kit is tailored for research applications in cancer immunology, immunotherapy development, and biomarker discovery.

Key Features

1. High Sensitivity and Specificity:
   • Analytical Sensitivity: <0.6 pg/mL.
   • Assay Range: 4.69–300 pg/mL.
   • Exclusively recognizes natural and recombinant human PD-L1.
2. Versatile Sample Compatibility:
   • Serum, plasma, cell lysates, and cell culture supernatants.
   • Requires only 50 µL per sample.
3. Efficient Workflow:
   • Hands-on Time: 1 hour 20 minutes.
   • Total Time-to-Result: 4 hours.
4. Reproducibility:
   • Intraassay CV: 2.1%.
   • Interassay CV: 3.4%.
5. Convenient Components:
   • Pre-coated 96-well plate.
   • Includes all necessary reagents for the assay, such as standards, detection antibodies, buffers, and chromogen.
6. Detection Method:
   • Instrument: Colorimetric Microplate Reader.
   • Detection System: HRP-based with chromogenic substrate.
7. Storage and Stability:
   • Store at 2–8°C.
   • Shipped on wet ice for optimal reagent integrity.

Principle of the Assay

The Human PD-L1 ELISA employs a solid-phase sandwich ELISA format:

1. A PD-L1-specific capture antibody is pre-coated on the plate wells.
2. Samples, standards, or controls are added to bind to the immobilized antibody.
3. A biotinylated detection antibody forms a sandwich with the captured PD-L1.
4. HRP-labeled streptavidin reacts with the substrate to generate a colorimetric signal proportional to the PD-L1 concentration.

Target Information

• Protein: PD-L1 (CD274)
• Aliases: B7-H1, PDCD1LG1, B7 homolog 1
• Function: Plays a key role in tumor immune evasion by binding to PD-1 on T cells, downregulating immune response.
• Applications:
  • Cancer immunology research.
  • Immune checkpoint pathway analysis.
  • Biomarker studies for immunotherapy development.
  • Investigating PD-L1 expression under inflammatory conditions (e.g., IFN-gamma stimulation).

Kit Components

1. Pre-coated 96-Well Plate: Immobilized PD-L1-specific capture antibody.
2. Standards: Calibrated PD-L1 standards for quantitative analysis.
3. Detection Antibody: Biotinylated, specific for PD-L1.
4. Buffers and Reagents:
   • Sample Diluent
   • Assay Buffer Concentrate
   • Wash Buffer Concentrate
   • HRP-labeled Streptavidin (SAV-HRP)
   • Chromogen Substrate
   • Stop Solution
5. Accessories:
   • Adhesive Plate Covers.

Applications in Research

1. Cancer Immunology:
   • Quantification of PD-L1 expression in tumor microenvironments.
   • Evaluation of PD-L1 levels in response to immune checkpoint inhibitors.
2. Immunotherapy Development:
   • Biomarker validation for immune checkpoint blockade therapies.
   • Monitoring the effectiveness of anti-PD-1/PD-L1 therapies.
3. Inflammation Studies:
   • Analysis of PD-L1 regulation by inflammatory cytokines like IFN-gamma.
   • Research on immune evasion mechanisms in chronic diseases.
4. Biomarker Discovery:
   • Identification of PD-L1 as a prognostic marker in oncology.

Product Specifications

Additional Information

• Shipping Conditions: Shipped on wet ice to maintain reagent integrity.
• Regulatory Status: For research use only (RUO); not for diagnostic use.

By delivering high sensitivity, accuracy, and versatility, the Invitrogen™ Human PD-L1 ELISA Kit is an essential tool for advancing research in immunology, oncology, and therapeutic development.