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Specifications:
| Application | Flow Cytometry | ||
| Storage Temperature | 2-8°C | ||
| Product Type | antibody | Forms | Liquid |
| Product Brand | BD Biosciences | ||
| Product Grade | Molecular Biology | ||
BD Horizon™ BV786 Mouse Anti-Human CD19 is a monoclonal antibody that specifically binds to CD19, a 90-kDa transmembrane glycoprotein expressed throughout B-cell development, excluding terminally differentiated plasma cells. CD19 is central to B-cell activation, development, and signal transduction, and is co-expressed with CD21 and CD81 on mature B cells. Its expression makes CD19 a critical marker for immunophenotyping and B-cell lineage studies.
This antibody is conjugated to BD Horizon™ Brilliant Violet 786 (BV786), a violet laser-excitable tandem dye with an emission peak at 786 nm. It is optimized for multicolor flow cytometry panels using a 405 nm laser and detectors compatible with Cy™7-like emission profiles (e.g., 780/60 filter).
Key Applications
- Flow Cytometry
- Human B-cell Detection and Subset Analysis
- Multicolor Immunophenotyping Panels
- Research in Immunology, Hematology, and Oncology
Fluorochrome Details
- Excitation: 405 nm
- Emission Max: 786 nm
- Filter Compatibility: 780/60 nm bandpass
- Fluorochrome Family: Brilliant Violet™
- Brilliant Stain Buffer Use: Recommended to prevent dye interactions
Technical Specifications
| Parameter | Details |
|---|---|
| Clone | SJ25C1 |
| Target Antigen | CD19 (Human) |
| Fluorochrome | BD Horizon™ BV786 |
| Application | Flow Cytometry |
| Volume per Test | 5 µL/test |
| Excitation Max | 407 nm |
| Emission Max | 786 nm |
| Buffer | Aqueous with BSA and ≤0.09% sodium azide |
| Storage Conditions | 2–8°C, protect from light, do not freeze |
| RRID | AB_2744314 |
| Alternative Names | B4, Leu-12, B-lymphocyte antigen CD19 |
| Immunogen | NALM1 + NALM16 human cell lines |
Recommended Use
- Sample Type: Human PBMCs, whole blood (processed), lymphoid tissues
- Isotype Control: Mouse IgG1, κ isotype at matched concentration
- Instrument Setup: Use BD’s Multicolor Flow Cytometry online tools for optimized configuration
Storage & Handling
- Store undiluted at 2–8°C
- Avoid prolonged light exposure
- Do not freeze
- Handle according to sodium azide safety protocols
The BD Horizon™ BV786 Mouse Anti-Human CD19 (SJ25C1) antibody is a powerful tool for identifying and analyzing B-cell populations in human samples. Its conjugation to BV786 ensures compatibility with modern multicolor flow cytometry systems, especially those using violet lasers. With proven specificity, brilliant emission characteristics, and regulatory-grade manufacturing standards, this reagent is a reliable component of any advanced immunophenotyping panel focused on B-cell biology, immunodeficiency, and malignancy research.
- Pack Size: For 100 Tests for 25 Tests