Thermo Scientific™ TAE Electrophoresis buffer solution 50X concentrate (TRIS-acetate-EDTA buffer)

The Thermo Scientific™ 50X TAE Buffer (Tris-acetate-EDTA) is a high-quality buffer solution designed for the electrophoresis of nucleic acids in both agarose and polyacrylamide gels. This buffer is suitable for genomic DNA, large supercoiled DNA, and RNA, making it versatile for a wide range of molecular biology applications. Its 50X concentrated formulation allows for easy dilution to the desired working concentration.

Key Features

1. Versatility:
   • Can be used as both a running buffer and a gel preparation buffer for electrophoresis.
   • Ideal for separating DNA fragments larger than 1500 bp, genomic DNA, and large supercoiled DNA.
2. High Purity:
   • Filtered through a 0.22 μm membrane, ensuring low levels of contaminants.
3. Ease of Use:
   • Supplied as a 50X concentrate, allowing flexible preparation of working solutions by dilution.
4. Compatibility:
   • Works effectively with both agarose gels and polyacrylamide gels.

Applications

1. Nucleic Acid Electrophoresis:
   • Separation of DNA and RNA in agarose or polyacrylamide gels for analysis or purification.
2. Genomic Studies:
   • Suitable for analyzing genomic DNA and large nucleic acid fragments.
3. Supercoiled DNA Analysis:
   • Ideal for resolving large supercoiled DNA, frequently used in plasmid studies.
4. Gel Preparation:
   • Used in gel casting and as an electrophoresis running buffer for optimal performance.

Specifications

Preparation Instructions

1. Dilution:
   • Dilute the 50X TAE concentrate to a 1X working solution using distilled water. For example:
     • Add 20 mL of 50X TAE buffer to 980 mL of distilled water to prepare 1 L of 1X TAE buffer.
2. Usage:
   • Use the diluted solution as the running buffer for nucleic acid electrophoresis or for gel preparation.

Advantages

• Efficient Separation:
  • Provides optimal resolution for nucleic acid fragments larger than 1500 bp.
• Flexible Applications:
  • Dual-purpose buffer for running gels and preparing gels.
• Convenient Packaging:
  • 50X concentration minimizes storage requirements and simplifies buffer preparation.
• Reliable Results:
  • Low contamination due to 0.22 μm filtration ensures reproducibility in sensitive experiments.

Important Notes

• Low Buffering Capacity:
  • TAE buffer has a relatively low buffering capacity compared to TBE (Tris-borate-EDTA). Replace the buffer periodically during prolonged electrophoresis runs to maintain performance.
• For Research Use Only:
  • Not intended for diagnostic procedures.

Storage

• Store the 50X TAE Buffer at room temperature or as recommended to maintain stability.

The Thermo Scientific™ 50X TAE Buffer provides a reliable and convenient solution for nucleic acid electrophoresis, ensuring optimal resolution and consistent results in genomic and molecular biology workflows.