Applied Biosystems™ TaqMan™ Multiplex Master Mix

Applied Biosystems™ TaqMan™ Multiplex Master Mix is a high-performance, 2X concentrated real-time PCR master mix designed to simultaneously amplify up to four targets in a single reaction. This master mix is optimized for gene expression and genotyping experiments, ensuring fast run times (<40 minutes), superior sensitivity, and high specificity.

It contains AmpliTaq™ Fast DNA Polymerase, uracil-N glycosylase (UNG) for carry-over contamination control, dNTPs with dUTP, MUSTANG PURPLE™ dye (passive reference), and an optimized buffer system. It is fully validated with TaqMan™ assays and TaqMan™ QSY™ probes, offering seamless integration into qPCR workflows.

Key Features & Benefits

1. Optimized for Multiplex qPCR (Up to 4 Targets)
   • Designed to simultaneously amplify multiple targets with high efficiency.
   • Ensures excellent concordance between singleplex and multiplex reactions.
2. Best-in-Class Performance
   • Provides superior sensitivity, accuracy, dynamic range, and specificity.
   • Benchmark-tested against leading multiplex qPCR master mixes.
3. Fast Cycling Conditions for Reduced Run Times
   • Optimized for fast cycling mode on standard and fast real-time PCR instruments.
   • Allows for significant time savings (<40-minute runs) while maintaining performance.
4. Carry-Over Contamination Control
   • Contains heat-labile uracil-DNA glycosylase (UDG) to prevent carry-over contamination.
   • Ensures amplification of only genuine target sequences.
5. Broad Compatibility with qPCR Instruments
   • Works on fast and standard qPCR platforms, including:
     • 7500 Real-Time PCR System
     • 7500 Fast Real-Time PCR System
     • ViiA™ 7 Real-Time PCR System
     • QuantStudio™ 5, 6 Flex, 7 Flex, 12K Flex

Product Specifications

Use Cases & Applications

1. Gene Expression & Molecular Biology Research

• Multiplex Gene Expression Analysis
  • Detects multiple gene transcripts in a single reaction.
  • Ideal for biomarker discovery and functional genomics studies.
• Quantification of Low-Abundance Transcripts
  • Enhances sensitivity and specificity of qPCR detection.
  • Ensures reproducible quantification of rare gene targets.

2. Viral Detection & Infectious Disease Research

• Multiplexed Virus Detection in Clinical Samples
  • Allows for simultaneous detection of multiple viral targets.
  • Used in epidemiological and diagnostic research studies.
• Rapid, High-Throughput qPCR Screening
  • Enables fast, accurate detection of viral RNA/DNA in patient samples.
  • Optimized for public health monitoring and pathogen surveillance.

3. SNP Genotyping & Mutation Analysis

• High-Throughput SNP Genotyping for Precision Medicine
  • Supports large-scale genotyping studies in genetic research.
  • Compatible with TaqMan™ QSY™ probes for allele discrimination.
• Mutational Analysis in Cancer Research
  • Identifies genetic variants associated with cancer progression.
  • Enables multiplex detection of oncogenic mutations.

4. Agricultural & Environmental qPCR Applications

• Plant Pathogen Detection & GMO Analysis
  • Detects multiple plant pathogens in a single assay.
  • Ensures efficient screening for genetically modified organisms (GMOs).
• Microbial Contaminant Testing in Water & Food Safety
  • Allows for simultaneous detection of bacterial and viral contaminants.
  • Ensures accurate environmental monitoring of microbial populations.

Optimized qPCR Workflow

Master Mix Preparation & Reaction Setup

1. Prepare the qPCR Reaction Mixture
   • Mix TaqMan™ Multiplex Master Mix (2X), primers, probes, and DNA template in appropriate volumes.
2. Load the Reaction Plate
   • Dispense the reaction mixture into qPCR-compatible 96- or 384-well plates.
3. Set Up the qPCR Instrumentation
   • Select fast cycling mode if using a compatible instrument.
4. Run the qPCR Reaction
   • Use a standard protocol with optimized annealing and extension temperatures.
5. Analyze the Data
   • Quantify amplification results using real-time PCR software.

Storage & Handling Instructions

• Storage Conditions:
  • Keep at 2–8°C (Refrigerated); DO NOT FREEZE.
  • Protect from light exposure to preserve reagent stability.
• Handling Precautions:
  • Use pipette tips with aerosol barriers to prevent contamination.
  • Work in a clean, DNA-free environment to avoid false positives.
• Disposal Guidelines:
  • Dispose of according to local biosafety and chemical waste disposal regulations.

Why Choose Applied Biosystems™ TaqMan™ Multiplex Master Mix?

✅ Validated for up to 4-target multiplex qPCR reactions

✅ Best-in-class sensitivity, specificity, and dynamic range

✅ Fast qPCR cycling mode for shorter run times (<40 minutes)

✅ Seamless compatibility with Applied Biosystems™ TaqMan™ assays

✅ Integrated UDG carry-over contamination control

Applied Biosystems™ TaqMan™ Multiplex Master Mix is a highly optimized qPCR reagent designed for high-throughput, high-precision multiplex qPCR applications, ensuring accurate gene expression analysis, genotyping, and pathogen detection.