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Applied Biosystems™ TaqMan™ Environmental Master Mix 2.0
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Specifications:
| Application | qPCR | ||
| Storage Temperature | -15°C to -25°C | ||
| Product Type | PCR Master Mix | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Applied Biosystems™ | ||
| Product Grade | Molecular Biology | ||
Applied Biosystems™ TaqMan™ Environmental Master Mix 2.0 is an advanced 2X qPCR master mix formulated specifically for accurate and reliable real-time PCR-based pathogen detection in environmental and food samples that often contain high levels of inhibitors. With optimized enzyme performance and ROX™ passive reference dye, this master mix provides exceptional sensitivity for low copy number targets, multiplex PCR, and quantification of viral and bacterial genetic material in challenging matrices.
Key Features
- Optimized for Inhibitor-Rich Samples – Delivers consistent, high-performance amplification even in complex environmental or food-derived samples.
- Ultra-Pure Enzyme System – Contains AmpliTaq Gold™ DNA Polymerase, UP (Ultra Pure) for superior specificity and low background signal.
- Built-In Hot Start Activation – Enables convenient room-temperature setup with enzyme activation during thermal cycling, minimizing non-specific amplification.
- Integrated ROX™ Reference Dye – Ensures precise fluorescence normalization and accurate quantification across qPCR platforms.
- High Sensitivity and Dynamic Range – Detects low copy number targets and supports multiplex assays with minimal cross-reactivity.
- Stable for High-Throughput Applications – Formulated for robustness and reproducibility in automated or large-scale workflows.
- Flexible Compatibility – Validated for use with Applied Biosystems™ 7000, 7300, 7500, 7500 Fast, 7900HT, and StepOne™ Real-Time PCR Systems.
Applications
- Quantitative pathogen detection in environmental monitoring
- Food safety testing for microbial contamination
- Viral and bacterial load quantification
- Gene expression and genotyping in complex matrices
- Water quality analysis and public health surveillance
- Research-based biosurveillance and molecular epidemiology
Technical Specifications
| Parameter | Description |
|---|---|
| Catalog Numbers | 4396838 (200 reactions), 4398021 (400 reactions), 4398044 (800 reactions) |
| Format | Bottle, 2X concentration |
| Polymerase | AmpliTaq Gold™ DNA Polymerase, UP (Ultra Pure) |
| Passive Reference Dye | ROX™ (pre-mixed) |
| Detection Method | Primer-probe (TaqMan™ chemistry) |
| Hot Start | Built-in (enzyme inactive at room temperature) |
| PCR Method | Quantitative PCR (qPCR) |
| Reaction Speed | Standard |
| Sample Type | Environmental and food samples |
| For Use With Equipment | ABI 7000, 7300, 7500, 7500 Fast, 7900HT, StepOne™ Systems |
| For Use With Applications | Gene Expression, Genotyping, Pathogen and Virus Detection |
| Purity Grade | UP (Ultra Pure) |
| Shipping Condition | Dry Ice |
| Storage | -15°C to -25°C (before first use), 2–8°C (after first use, protected from light) |
| Product Line | TaqMan™ |
The Applied Biosystems™ TaqMan™ Environmental Master Mix 2.0 combines the proven reliability of AmpliTaq Gold™ polymerase with enhanced inhibitor tolerance and precision data normalization. Designed for high-sensitivity, quantitative pathogen detection in challenging environmental and food matrices, it ensures reproducible results, reduced background, and compatibility with a broad range of Applied Biosystems™ real-time PCR platforms. This ready-to-use 2X master mix is ideal for research laboratories engaged in environmental, food, and public health molecular testing.
- Pack Size: 1 x 5 mL, sufficient for 200 Rxns 2 x 5 mL, sufficient for 400 Rxns 4 x 5 mL, sufficient for 800 Rxns