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IPTG is a high-purity reagent widely used in molecular biology, specifically for inducing expression in cloning experiments and identifying recombinant colonies. Its dioxane-free formulation ensures compatibility with sensitive applications.
Key Features
- High Purity:
- Purity of ≥99%, ensuring reproducibility in molecular biology workflows.
- Dioxane-Free:
- Free of dioxane contaminants, making it ideal for critical and sensitive experiments.
- Colorimetric Detection:
- Used alongside X-Gal in blue/white screening for visual identification of recombinants in cloning strategies.
Chemical and Physical Properties
| Property | Details |
|---|---|
| Chemical Name | Isopropyl-β-D-thiogalactopyranoside |
| Synonyms | IPTG, (2R,3R,4S,5R,6S)-2-(Hydroxymethyl)-6-(isopropylthio)tetrahydro-2H-pyran-3,4,5-triol |
| Molecular Formula | C₉H₁₈O₅S |
| Molecular Weight | 238.31 g/mol |
| CAS Number | 367-93-1 |
| EINECS Number | 206-703-0 |
| MDL Number | MFCD00063273 |
| Melting Point | 105 °C |
| Storage Temperature | Refrigerator (2–8 °C) |
Applications
- Gene Expression:
- IPTG is used as an inducer for the lac operon in bacterial systems.
- Induces expression of genes under the control of lac operator/promoter systems.
- Cloning and Screening:
- Combined with X-Gal for blue/white screening to differentiate between recombinant (white) and non-recombinant (blue) colonies in bacterial transformation.
- Protein Production:
- Induces high levels of recombinant protein expression in systems using IPTG-inducible promoters.
- Molecular Biology Research:
- Widely used in experimental setups involving controlled gene expression in bacterial systems.
Advantages
- High Purity: ≥99% assay ensures minimal contaminants for precise molecular biology applications.
- Dioxane-Free: Safe for experiments requiring minimal chemical interference.
- Reliable Induction: Proven effectiveness in systems using the lac operon for controlled gene expression.
- Stable and Easy to Use: Melting point of 105 °C and storage at 2–8 °C ensure stability.
Specifications
| Test Parameter | Specification |
|---|---|
| Purity (Assay) | ≥99% |
| Physical Form | Crystalline Powder |
| Melting Point | 105 °C |
| Storage Conditions | Refrigerated (2–8 °C) |
Storage and Handling
- Storage Temperature: Store in a refrigerator (2–8 °C) to maintain stability.
- Packaging: Supplied in tightly sealed containers to prevent moisture absorption.
- Shelf Life: Stable under recommended storage conditions.
Safety Information
- Hazard Classification: Irritant; avoid contact with skin and eyes.
- Precautions:
- Use gloves, goggles, and protective clothing when handling.
- Handle in a well-ventilated area to avoid inhalation of powder.
- Disposal: Dispose of in accordance with local chemical waste regulations.
Apollo Scientific IPTG (Isopropyl-β-D-thiogalactopyranoside) is a high-purity, dioxane-free reagent tailored for gene expression and blue/white screening in molecular biology research. Its reliability and compatibility with sensitive experiments make it a critical tool for bacterial transformation and protein production workflows.
No resources are currently available for this product.
IPTG is a high-purity reagent widely used in molecular biology, specifically for inducing expression in cloning experiments and identifying recombinant colonies. Its dioxane-free formulation ensures compatibility with sensitive applications.
Key Features
- High Purity:
- Purity of ≥99%, ensuring reproducibility in molecular biology workflows.
- Dioxane-Free:
- Free of dioxane contaminants, making it ideal for critical and sensitive experiments.
- Colorimetric Detection:
- Used alongside X-Gal in blue/white screening for visual identification of recombinants in cloning strategies.
Chemical and Physical Properties
| Property | Details |
|---|---|
| Chemical Name | Isopropyl-β-D-thiogalactopyranoside |
| Synonyms | IPTG, (2R,3R,4S,5R,6S)-2-(Hydroxymethyl)-6-(isopropylthio)tetrahydro-2H-pyran-3,4,5-triol |
| Molecular Formula | C₉H₁₈O₅S |
| Molecular Weight | 238.31 g/mol |
| CAS Number | 367-93-1 |
| EINECS Number | 206-703-0 |
| MDL Number | MFCD00063273 |
| Melting Point | 105 °C |
| Storage Temperature | Refrigerator (2–8 °C) |
Applications
- Gene Expression:
- IPTG is used as an inducer for the lac operon in bacterial systems.
- Induces expression of genes under the control of lac operator/promoter systems.
- Cloning and Screening:
- Combined with X-Gal for blue/white screening to differentiate between recombinant (white) and non-recombinant (blue) colonies in bacterial transformation.
- Protein Production:
- Induces high levels of recombinant protein expression in systems using IPTG-inducible promoters.
- Molecular Biology Research:
- Widely used in experimental setups involving controlled gene expression in bacterial systems.
Advantages
- High Purity: ≥99% assay ensures minimal contaminants for precise molecular biology applications.
- Dioxane-Free: Safe for experiments requiring minimal chemical interference.
- Reliable Induction: Proven effectiveness in systems using the lac operon for controlled gene expression.
- Stable and Easy to Use: Melting point of 105 °C and storage at 2–8 °C ensure stability.
Specifications
| Test Parameter | Specification |
|---|---|
| Purity (Assay) | ≥99% |
| Physical Form | Crystalline Powder |
| Melting Point | 105 °C |
| Storage Conditions | Refrigerated (2–8 °C) |
Storage and Handling
- Storage Temperature: Store in a refrigerator (2–8 °C) to maintain stability.
- Packaging: Supplied in tightly sealed containers to prevent moisture absorption.
- Shelf Life: Stable under recommended storage conditions.
Safety Information
- Hazard Classification: Irritant; avoid contact with skin and eyes.
- Precautions:
- Use gloves, goggles, and protective clothing when handling.
- Handle in a well-ventilated area to avoid inhalation of powder.
- Disposal: Dispose of in accordance with local chemical waste regulations.
Apollo Scientific IPTG (Isopropyl-β-D-thiogalactopyranoside) is a high-purity, dioxane-free reagent tailored for gene expression and blue/white screening in molecular biology research. Its reliability and compatibility with sensitive experiments make it a critical tool for bacterial transformation and protein production workflows.
No resources are currently available for this product.
Specifications
| Pack Size | 5g, 10g, 25g |