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![Apollo Sci. HEPES (2-[4-(2-hydroxyethyl)-1-piperazinyl]ethanesulphonic acid) free acid ≥99.5%, Ultrapure](/web/image/product.product/46626/image_1024/%5BBI8181-250G%5D%20Apollo%20Sci.%20HEPES%20%282-%5B4-%282-hydroxyethyl%29-1-piperazinyl%5Dethanesulphonic%20acid%29%20free%20acid%20%E2%89%A599.5%25%2C%20Ultrapure%20%28250g%29?unique=5055fcd)
Apollo Sci. HEPES (2-[4-(2-hydroxyethyl)-1-piperazinyl]ethanesulphonic acid) free acid ≥99.5%, Ultrapure
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Specifications:
| Application | molecular biology | ||
| Storage Temperature | Ambient | ||
| Product Type | Buffer | Forms | Powder |
| Product Brand | Apollo Scientific | ||
| Product Grade | Molecular Biology | Formula | C₈H₁₈N₂O₄S |
HEPES (2-[4-(2-hydroxyethyl)-1-piperazinyl]ethanesulphonic acid) is a high-purity zwitterionic buffering agent widely used in cell culture, molecular biology, biochemistry, diagnostic kit manufacturing, and protein research. With a pKa of 7.55 ± 0.2 at 20 °C, HEPES provides exceptional buffering capacity within its useful biological range of pH 6.8–8.2, making it ideal for applications requiring stable, CO₂-independent pH control.
Apollo Sci. HEPES free acid is produced at ≥99.5% purity and meets stringent specifications for biological and biochemical research. Its very low heavy metal content (<1 ppm) ensures excellent compatibility with sensitive enzymatic systems, mammalian cell culture, nucleic acid work, and high-resolution analytical procedures.
Key Features
- Ultrapure grade (≥99.5%) for high-sensitivity biological and biochemical applications
- Highly effective buffer with a useful pH range of 6.8–8.2
- pKa 7.55 ± 0.2 at 20°C, supporting stable pH environments without CO₂
- Very low heavy metal contamination (<1 ppm)
- Exceptional solubility and compatibility with enzymes, proteins, antibodies, and cell culture media
- Manufactured to support reproducibility in demanding research and diagnostic workflows
- Available in 100 g, 250 g, and 1 kg pack sizes
Synonyms
- HEPES free acid
- N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulphonic acid)
- 4-(2-Hydroxyethyl)piperazin-1-ylethanesulphonic acid
- N-(2-Hydroxyethyl)piperazine-N′-2-ethanesulphonic acid
- 2-(4-(2-Hydroxyethyl)piperazin-1-yl)ethanesulphonic acid
Applications
- Cell culture media buffering—stable pH without CO₂ incubation
- Protein purification and stabilization
- Electrophoresis, chromatography, and nucleic acid workflows
- Enzymatic reactions sensitive to pH fluctuations
- Diagnostic reagent and kit formulation
- Immunoassays and biological manufacturing processes
Technical Specifications
| Property | Value |
|---|---|
| Purity | ≥99.5% |
| CAS Number | 7365-45-9 |
| Chemical Formula | C₈H₁₈N₂O₄S |
| Molecular Weight | 238.31 g/mol |
| MDL Number | MFCD00006158 |
| EINECS | 230-907-9 |
| Melting Point | 238–240 °C |
| Heavy Metals | <1 ppm |
| pKa (20°C) | 7.55 ± 0.2 |
| Useful pH Range | 6.8–8.2 |
| Storage Temperature | Ambient |
| Form | Free acid powder |
| Chemical Type | Zwitterionic biological buffer |
Ordering Information
| Catalog Number | Pack Size | Packaging |
|---|---|---|
| BI8181-100G | 100 g | Bottle |
| BI8181-250G | 250 g | Bottle |
| BI8181-1KG | 1 kg | Glass bottle |
Apollo Sci. HEPES Free Acid ≥99.5% is a premium ultrapure buffer engineered for the precise and demanding needs of modern biological research. With excellent purity, low metal content, and optimal pH characteristics, it provides unmatched reliability in cell culture, enzymology, nucleic acid workflows, and diagnostic reagent formulation. Its stability, reproducibility, and compatibility with sensitive assays make it an essential component for laboratories seeking consistent, high-quality results.
- Pack Size: 250g 1kg 100g