Abcam Human Neurofilament H ELISA Kit (200 kDa Neurofilament protein)

The Abcam Human Neurofilament H ELISA Kit (200 kDa Neurofilament Protein) is a high-performance tool designed for the quantitative measurement of Human Neurofilament H (NF-H) in tissue extracts and cell lysate samples. Utilizing the SimpleStep ELISA® technology, this single-wash protocol delivers accurate results in just 90 minutes, making it a convenient and reliable option for research applications.

Target Overview: Neurofilament H (200 kDa)

• Function:
  • Neurofilament H (NF-H) is a key structural component of the neuronal cytoskeleton, playing a critical role in maintaining the caliber and stability of axons.
  • It interacts with other intermediate filament proteins, including NEFL (light chain) and NEFM (medium chain), to form neurofilaments crucial for proper neuronal function.
  • NF-H is particularly significant in mature axons and may also interact with PRPH and INA to create filamentous neuronal networks.
• Alternative Names:
  • NF-H
  • Neurofilament heavy polypeptide
  • 200 kDa neurofilament protein
  • Neurofilament triplet H protein

Kit Features and Benefits

• Simplified Workflow:
  • Single-wash, 90-minute assay for time-efficient processing.
• High Specificity:
  • Targets Human Neurofilament H (NF-H) with precision, ensuring minimal cross-reactivity.
• Broad Sample Compatibility:
  • Suitable for analyzing tissue extracts and cell lysates.
• Comprehensive Kit Components:
  • Includes pre-coated 96-well microplate and all necessary reagents for a complete assay.

Applications

• Neuroscience Research:
  • Study axonal structure and function in neuronal systems.
  • Investigate neurodegenerative diseases, such as ALS, Parkinson’s disease, or Alzheimer’s disease, where neurofilament levels are biomarkers for neuronal damage.
• Cell Biology:
  • Analyze the role of neurofilaments in maintaining cytoskeletal integrity.
  • Examine NF-H dynamics during neuronal differentiation and axonal injury.
• Protein Quantification:
  • Quantify NF-H expression levels in various cell and tissue models.
• Drug Discovery:
  • Evaluate the effects of therapeutic compounds on neuronal health and axonal stability.

Kit Components

1. 10X Human Neurofilament H (Capture Antibody) – 600 µL
2. 10X Human Neurofilament H (Detector Antibody) – 600 µL
3. 10X Wash Buffer PT (ab206977) – 20 mL
4. 50X Cell Extraction Enhancer Solution (ab193971) – 1 mL
5. 5X Cell Extraction Buffer PTR (ab193970) – 10 mL
6. Antibody Diluent 5BI – 6 mL
7. Human Neurofilament H Lyophilized Recombinant Protein – 2 vials
8. Plate Seals – 1 unit
9. Sample Diluent NS (ab193972) – 12 mL
10. SimpleStep Pre-Coated 96-Well Microplate (ab206978) – 1 unit
11. Stop Solution – 12 mL
12. TMB Development Solution – 12 mL

Technical Specifications

• Analytical Sensitivity: High sensitivity for precise quantification.
• Assay Duration: 90 minutes.
• Assay Range: Reliable detection across a broad range of NF-H concentrations.
• Sample Volume: Requires only 50 µL per sample.
• Instrument Compatibility: Requires a colorimetric microplate reader.

Shipping and Storage

• Shipping Conditions: Shipped on wet ice to maintain reagent stability.
• Storage: Store at 2–8°C as per component-specific guidelines to ensure longevity and performance.

Use Cases

1. Quantifying Axonal Injury:
   • Assess NF-H levels as a biomarker for axonal damage in neurodegenerative diseases.
2. Drug Efficacy Testing:
   • Monitor changes in NF-H expression in response to neuroprotective treatments.
3. Neuronal Differentiation Studies:
   • Evaluate NF-H as an indicator of mature neuronal phenotypes.
4. Cytoskeletal Dynamics:
   • Investigate how alterations in neurofilament composition affect neuronal structure and function.

Notes

• For Research Use Only: Not for use in diagnostic procedures.
• Validation: Each kit lot undergoes rigorous testing for sensitivity, specificity, and reproducibility.

This ELISA kit is a valuable tool for neuroscience and cell biology researchers aiming to unravel the complexities of neuronal structure and neurodegeneration.