Proteintech™ Atl Blue Anti-Human CD21 (BU32), 100 Tests

The Proteintech™ Atl Blue Anti-Human CD21 (BU32) antibody is a premium monoclonal antibody designed for detecting CD21 (Complement Receptor 2, CR2) in human cells. This antibody is conjugated to Atl Blue, a bright and photostable fluorochrome, optimized for flow cytometry applications. CD21, a type I transmembrane protein, plays a crucial role in immune responses, serving as a receptor for complement component C3d and Epstein-Barr virus (EBV). This reagent is validated for 100 tests, offering researchers a reliable tool for immunophenotyping and functional studies.

Key Features:

1. Target and Specificity:
   • Recognizes CD21, a 145 kDa protein expressed on mature B cells, follicular dendritic cells, and subsets of epithelial cells.
   • Serves as a key marker for studying B-cell biology and complement receptor activity.
2. Clone and Conjugate:
   • Clone: BU32, providing high specificity and sensitivity.
   • Conjugate: Atl Blue, a bright and photostable fluorochrome ideal for flow cytometry.
3. Validated Applications:
   • Designed and tested for flow cytometric analysis of human peripheral blood cells and other human samples.
4. High Performance:
   • Bright fluorescence emission ensures strong signal detection and low background.
   • Photostable fluorochrome maintains signal integrity during extended experimental protocols.
5. Format:
   • Supplied as a ready-to-use liquid formulation for flow cytometry applications.
   • Includes 100 tests, with each test sufficient for staining approximately 10⁶ cells.

Specifications:

Applications:

1. Flow Cytometry:
   • Suitable for analyzing CD21 expression on human B cells, follicular dendritic cells, and epithelial cells.
2. Immunophenotyping:
   • Identify and characterize subsets of B cells in clinical and research samples.
3. Functional Studies:
   • Investigate CD21's role in immune responses, including its interaction with complement components and EBV.
4. Complement System Research:
   • Study the role of CD21 in complement activation and immune complex retention.

Key Benefits:

1. Superior Brightness:
   • Atl Blue provides high-intensity fluorescence for easy detection in flow cytometry panels.
2. High Specificity and Sensitivity:
   • BU32 clone ensures minimal background and accurate detection of CD21-expressing cells.
3. Ready-to-Use:
   • Pre-formulated liquid solution simplifies workflows and ensures consistent results.
4. Photostability:
   • Atl Blue fluorochrome maintains signal integrity throughout experimental procedures.
5. Reproducible Results:
   • Validated for flow cytometry, ensuring consistent and reliable performance.

Recommended Usage:

1. Stain approximately 10⁶ cells per test in 100 µL of staining buffer.
2. Incubate with the antibody solution as per the recommended protocol.
3. Analyze using a flow cytometer equipped with a 488 nm blue laser.

Storage and Handling:

• Store at 2–8°C and protect from prolonged exposure to light.
• Avoid repeated freeze-thaw cycles to maintain reagent quality and efficacy.

For Research Use Only:

This product is intended for research purposes only and is not for use in diagnostic or therapeutic procedures.