Invitrogen™ CytKick™ Autosampler

Automated High-Throughput Sample Loading for the Attune™ NxT Flow Cytometer

The Invitrogen™ CytKick™ Autosampler (Catalog No. A42901) is an advanced automation accessory designed exclusively for the Attune™ NxT Acoustic Focusing Cytometer. It enables high-throughput acquisition of flow cytometry data from 96- and 384-well plates (both standard and deep formats), increasing lab productivity while maintaining sample quality. With intelligent engineering focused on reliability, flexibility, and ease of use, the CytKick Autosampler is essential for researchers conducting screening workflows, immune profiling, or multiparametric cytometry with large sample sets.

Key Features and Benefits

• Reliable Intelligent Probe Design
  Equipped with a 200 µm diameter probe, the CytKick minimizes clogging and carryover (≤0.5%). It features automated self-calibration to prevent probe crashes and ensure consistent alignment across wells.
• Mixing by Aspiration
  Ensures homogeneous sample resuspension without compromising cell viability. This mixing method provides consistent results with CVs under 10% across the plate—superior to traditional shaker-based systems.
• Plate Compatibility
  Supports 96-well, 384-well, and deep-well microplate formats, accommodating a wide variety of assay types and workflows.
• Easy Switching Between Plate and Tube Modes
  No disassembly, rebooting, or QC reruns required. Software-detected mode switching ensures seamless workflow transition and full accessibility to the manual sample probe.
• Automated Maintenance
  Integrated auto-cleaning cycle activates during instrument shutdown, ensuring clean operation with minimal user input.
• User-Friendly Interface
  The intuitive Attune NxT Cytometric Software features a plate layout editor, instrument settings wizard, compensation well design, and real-time sample acquisition heat maps for effortless setup and analysis.
• Visual System Monitoring
  Includes fluid bottle sensors and indicator lights to communicate system status, even when the operator is away from the workstation.

Application Highlights

• High-throughput flow cytometry for drug screening, biomarker profiling, or cell subset analysis
• Multiparametric assays in immunology, oncology, stem cell research, and microbiology
• Automated acquisition from microplates without compromising sample integrity
• Consistent and reproducible data across large sample cohorts
• Ideal for walk-away operation in busy core labs or translational research facilities

Technical Specifications

Kit Contents

• 1 × CytKick Autosampler
• 1 × Installation Kit
• 1 × Pack of 10 Standard 96-Well Plates

The Invitrogen™ CytKick™ Autosampler provides high-throughput, automated sample loading with unmatched reliability and ease of use. Its aspiration mixing, intelligent self-calibrating probe, and automated cleaning routines support consistent performance and minimal downtime in demanding flow cytometry applications. Whether you're performing immune monitoring, drug screening, or multiplexed cytometry in a 96- or 384-well plate format, CytKick streamlines your workflow and enhances your lab's productivity.