Gibco™ YPD Broth, 1L

The Gibco™ YPD Broth is a sterile, ready-to-use liquid medium designed for yeast culture, specifically for the growth and propagation of Saccharomyces cerevisiae (S. cerevisiae) and Pichia pastoris (P. pastoris) strains. Ideal for applications like protein expression, cloning, and yeast cell expansion, this filter-sterilized medium comes in an ergonomic bottle for easy handling and precise pipetting.

Key Features:

1. Ready-to-Use Liquid Media:
   • Eliminates the need for preparation, ensuring convenience and consistency.
2. Optimized for Yeast Cultivation:
   • Supports the maintenance and propagation of S. cerevisiae and P. pastoris, among other yeast strains.
3. Sterile and Reliable:
   • Filter-sterilized to prevent contamination and ensure consistent experimental results.
4. Ergonomic Bottle Design:
   • Features an angled opening for simplified pipetting and reduced spillage.

Specifications:

Formulation per Liter:

• Yeast Extract: 10 g
• Bacto Peptone: 20 g
• Dextrose (Glucose): 20 g

Applications:

1. Yeast Maintenance and Propagation:
   • Ideal for routine yeast culturing and maintenance.
2. Protein Expression Studies:
   • Supports the production of recombinant proteins in yeast expression systems.
3. Cloning and Gene Editing:
   • Enables yeast-based molecular cloning experiments.
4. Yeast Expansion:
   • Scalable for high-yield yeast cultures in research applications.
5. Microbial Genetics Research:
   • Suitable for studies involving yeast genetics and transformation.

Advantages:

1. Convenience:
   • Eliminates time-consuming media preparation with a ready-to-use format.
2. Consistent Performance:
   • Sterile filtration ensures reproducible results and minimizes contamination risks.
3. Ergonomic Design:
   • Angled bottle openings make pipetting easy and reduce the risk of contamination.
4. Reliable Formulation:
   • Optimized for robust growth of yeast strains, ensuring high-density cultures.

Preparation Instructions:

1. Use Directly:
   • No additional preparation is required; simply open the bottle and use the media for yeast cultures.
2. Inoculate Yeast:
   • Transfer the yeast inoculum into the YPD Broth for cultivation.
3. Incubate:
   • Maintain cultures at the appropriate temperature (typically 30°C) and shaking speed (e.g., 200 rpm) for optimal growth.

Ordering Information:

Storage and Handling:

• Storage Conditions: Store at 2–8°C in a dry, dark environment.
• Shelf Life: Refer to product labeling for expiration date.

Related Products:

• Gibco™ Yeast Extract Powder: For custom yeast media preparation.
• Gibco™ YPD Agar: For solid-phase yeast culture.
• Gibco™ DMEM/F-12 Medium: For other microbial or mammalian cell culture needs.

The Gibco™ YPD Broth offers a convenient, ready-to-use solution for yeast cultivation in a variety of research applications. Its sterile formulation, optimized composition, and ease of use make it an ideal choice for laboratories requiring consistent and reliable yeast growth media.