Liofilchem® Ceftaroline CPT, Antimicrobial Susceptibility Discs, 30 µg

The Liofilchem® Ceftaroline (CPT) Antimicrobial Susceptibility Discs, 30 µg are designed for in vitro disc diffusion testing to determine the susceptibility of bacteria to ceftaroline, a fifth-generation cephalosporin. Ceftaroline is active against a range of Gram-positive and some Gram-negative organisms and is notable for its effectiveness against methicillin-resistant Staphylococcus aureus (MRSA).

Each disc contains 30 µg of ceftaroline, formulated according to CLSI and EUCAST standards, and is used in standardized antimicrobial susceptibility testing procedures using Mueller-Hinton agar.

These discs are widely used in clinical microbiology laboratories, hospital infection control, research, and antimicrobial surveillance programs, especially when testing for multidrug-resistant pathogens.

Key Features

• High-quality 30 µg potency per disc, meeting international AST standards.
• Reliable detection of ceftaroline susceptibility, especially for MRSA and other multidrug-resistant Gram-positive organisms.
• Certified manufacturing under ISO 9001 and ISO 13485 quality management systems.
• Ready-to-use discs individually sealed in aluminum blister packs with desiccant for optimal stability.
• Compatible with manual and automated disc dispensers and standard AST workflows.

Specifications

Use Cases

1. Detection of MRSA Susceptibility to Ceftaroline

Ceftaroline is one of the few β-lactams effective against MRSA. These discs are routinely used to assess S. aureus, especially methicillin-resistant strains, for ceftaroline susceptibility to guide therapy in serious infections such as:

• Bloodstream infections
• Pneumonia
• Complicated skin and soft tissue infections

2. Testing of Multidrug-Resistant Streptococci and Gram-negative Isolates

While ceftaroline has primary activity against Gram-positive cocci, it is also tested against select Gram-negative organisms such as Haemophilus influenzae and E. coli, especially in:

• Respiratory samples
• Surgical site infections
• Nosocomial pathogen surveillance

3. Antibiogram Development and Resistance Surveillance

• Used by microbiology labs to update local antibiograms.
• Integrated into hospital stewardship programs to monitor resistance trends and inform empirical prescribing policies.

4. Research and Drug Development

Ideal for academic and clinical researchers investigating:

• Resistance evolution in MRSA
• Ceftaroline activity across diverse bacterial species
• Synergy testing with other β-lactams or inhibitors

Testing Procedure

1. Prepare the Inoculum
   • Prepare a 0.5 McFarland standard suspension of the test organism using sterile saline or broth.
2. Inoculate the Mueller-Hinton Agar Plate
   • Swab the surface of the agar uniformly with the inoculum using a sterile swab.
3. Apply the Ceftaroline Disc (30 µg)
   • Place one disc on the agar surface using sterile forceps or a disc dispenser.
   • Ensure discs are firmly in contact with the agar and evenly spaced.
4. Incubate the Plate
   • Invert and incubate at 35 ± 2 °C for 16–20 hours in ambient air.
   • Use 5% CO₂ for Streptococcus pneumoniae or other fastidious organisms if needed.
5. Measure and Interpret the Zone of Inhibition
   • Measure the diameter of the zone (in mm) with a ruler or caliper.
   • Compare zone size to CLSI or EUCAST breakpoint tables for interpretation:
     • Susceptible (S)
     • Intermediate (I)
     • Resistant (R)

Interpretation Standards (Reference – Always Check Latest CLSI/EUCAST)

Note: Always consult the current year CLSI or EUCAST documentation for accurate interpretive criteria.

Comparison with Related AST Discs

Unique Advantages of Liofilchem® Ceftaroline Discs

• Precision Formulation: Delivers consistent diffusion and accurate zone measurements.
• Regulatory Compliance: Meets international AST standards for clinical use.
• Extended Shelf Life: Stable for 24 months when stored correctly.
• Broad Application: Effective for routine diagnostics, surveillance, and academic research.
• Trusted Manufacturer: Produced by Liofilchem®, a globally recognized provider of IVD microbiology products.

For In Vitro Diagnostic Use Only. Not for therapeutic use.