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The Liofilchem® Meropenem+Phenylboronic Acid (MR+BO) Antimicrobial Susceptibility Discs are designed to detect Klebsiella pneumoniae carbapenemase (KPC) production and other class A beta-lactamase activity in Gram-negative bacteria, including Enterobacterales. These discs combine Meropenem with Phenylboronic Acid (PBA), an inhibitor of KPC enzymes, for reliable detection of carbapenem-resistant strains due to KPC production.
Key Features
- Specific Detection:
- Identifies KPC-producing bacteria by observing the synergistic effect of PBA in restoring meropenem activity.
- Dual Disc System:
- Includes separate discs for Meropenem (MR) and Meropenem+Phenylboronic Acid (MR+BO) for comparative zone diameter analysis.
- Accurate Results:
- A ≥5 mm increase in the zone of inhibition with MR+BO compared to MR alone confirms KPC activity.
- Ease of Use:
- Compatible with the standardized disc diffusion method on Mueller-Hinton agar.
- Clinical Relevance:
- Essential for guiding antimicrobial therapy in infections caused by carbapenem-resistant pathogens.
Applications
| Application | Description |
|---|---|
| Clinical Diagnostics | Detects KPC production in Gram-negative pathogens. |
| Antimicrobial Resistance Studies | Monitors the prevalence of KPC-producing bacteria in healthcare settings. |
| Pharmaceutical Research | Evaluates carbapenem+PBA combination therapies against resistant strains. |
Testing Procedure
- Preparation:
- Prepare a bacterial suspension to 0.5 McFarland standard.
- Spread the suspension uniformly on Mueller-Hinton agar plates.
- Application:
- Place the Meropenem (MR) and Meropenem+PBA (MR+BO) discs on the agar surface using sterile forceps.
- Incubation:
- Incubate plates at 35–37°C for 16–20 hours under aerobic conditions.
- Interpretation:
- Measure and compare the zones of inhibition for both discs:
- A zone diameter increase of ≥5 mm with MR+BO indicates KPC production.
- Measure and compare the zones of inhibition for both discs:
Storage and Stability
| Condition | Temperature | Duration |
|---|---|---|
| Long-Term Storage | -20°C | Until expiration date |
| Short-Term Storage | 2–8°C | Up to 2 weeks |
| During Use | Room Temperature | Immediate use only |
Technical Specifications
| Feature | Details |
|---|---|
| Active Components | Meropenem (10 µg) and Meropenem+Phenylboronic Acid (10 µg + PBA) |
| Test Method | Disc diffusion |
| Compatible Media | Mueller-Hinton agar |
| Incubation Conditions | 35–37°C for 16–20 hours |
| Target Pathogens | KPC-producing Gram-negative bacteria |
| Shelf Life | As per expiration date on packaging |
| Pack Sizes | 50 or 100 discs per pack |
Quality Control Data
| Control Organism | Expected Results |
|---|---|
| Escherichia coli ATCC® 25922 | MR: ≥23 mm; MR+BO: Similar |
| Klebsiella pneumoniae (KPC-positive strain) | MR: Reduced zone; MR+BO: Zone diameter increased by ≥5 mm |
| Pseudomonas aeruginosa ATCC® 27853 | MR: ≥18 mm; MR+BO: Similar |
| Staphylococcus aureus ATCC® 29213 | Not applicable (Gram-positive control) |
- Positive Controls: Confirm detection of KPC-producing strains.
- Negative Controls: Ensure no false-positive results in non-KPC producers.
Interpretation of Results
| Zone of Inhibition Comparison | Interpretation |
|---|---|
| MR zone = MR+BO zone | Absence of KPC production. |
| MR zone ≥5 mm smaller than MR+BO zone | Presence of KPC-producing bacteria. |
Comparison with Other Disc-Based Tests
| Test Disc | Recommended Use |
|---|---|
| Meropenem+Phenylboronic Acid (MR+BO) | Specific detection of KPC beta-lactamase producers. |
| Meropenem+EDTA (MR+ED) | Detection of metallo-beta-lactamase (MBL) producers. |
| Meropenem+Cloxacillin (MR+CL) | Detection of AmpC beta-lactamase producers. |
| Carbapenemase Combination Discs | General detection of carbapenemase-mediated resistance. |
Advantages
| Feature | Benefit |
|---|---|
| Dual Testing System | Differentiates KPC resistance from other carbapenemases. |
| High Sensitivity and Specificity | Reduces false-positive and false-negative results. |
| Ease of Use | Simplified setup minimizes errors and time. |
| Standards Compliance | Adheres to global antimicrobial susceptibility testing guidelines. |
Conclusion
The Liofilchem® Meropenem+Phenylboronic Acid (MR+BO) Antimicrobial Susceptibility Discs offer a reliable and precise method for detecting KPC-mediated carbapenem resistance in Gram-negative bacteria. Their high sensitivity, specificity, and adherence to international testing standards make them essential for diagnostic laboratories and resistance monitoring programs.
No resources are currently available for this product.
The Liofilchem® Meropenem+Phenylboronic Acid (MR+BO) Antimicrobial Susceptibility Discs are designed to detect Klebsiella pneumoniae carbapenemase (KPC) production and other class A beta-lactamase activity in Gram-negative bacteria, including Enterobacterales. These discs combine Meropenem with Phenylboronic Acid (PBA), an inhibitor of KPC enzymes, for reliable detection of carbapenem-resistant strains due to KPC production.
Key Features
- Specific Detection:
- Identifies KPC-producing bacteria by observing the synergistic effect of PBA in restoring meropenem activity.
- Dual Disc System:
- Includes separate discs for Meropenem (MR) and Meropenem+Phenylboronic Acid (MR+BO) for comparative zone diameter analysis.
- Accurate Results:
- A ≥5 mm increase in the zone of inhibition with MR+BO compared to MR alone confirms KPC activity.
- Ease of Use:
- Compatible with the standardized disc diffusion method on Mueller-Hinton agar.
- Clinical Relevance:
- Essential for guiding antimicrobial therapy in infections caused by carbapenem-resistant pathogens.
Applications
| Application | Description |
|---|---|
| Clinical Diagnostics | Detects KPC production in Gram-negative pathogens. |
| Antimicrobial Resistance Studies | Monitors the prevalence of KPC-producing bacteria in healthcare settings. |
| Pharmaceutical Research | Evaluates carbapenem+PBA combination therapies against resistant strains. |
Testing Procedure
- Preparation:
- Prepare a bacterial suspension to 0.5 McFarland standard.
- Spread the suspension uniformly on Mueller-Hinton agar plates.
- Application:
- Place the Meropenem (MR) and Meropenem+PBA (MR+BO) discs on the agar surface using sterile forceps.
- Incubation:
- Incubate plates at 35–37°C for 16–20 hours under aerobic conditions.
- Interpretation:
- Measure and compare the zones of inhibition for both discs:
- A zone diameter increase of ≥5 mm with MR+BO indicates KPC production.
- Measure and compare the zones of inhibition for both discs:
Storage and Stability
| Condition | Temperature | Duration |
|---|---|---|
| Long-Term Storage | -20°C | Until expiration date |
| Short-Term Storage | 2–8°C | Up to 2 weeks |
| During Use | Room Temperature | Immediate use only |
Technical Specifications
| Feature | Details |
|---|---|
| Active Components | Meropenem (10 µg) and Meropenem+Phenylboronic Acid (10 µg + PBA) |
| Test Method | Disc diffusion |
| Compatible Media | Mueller-Hinton agar |
| Incubation Conditions | 35–37°C for 16–20 hours |
| Target Pathogens | KPC-producing Gram-negative bacteria |
| Shelf Life | As per expiration date on packaging |
| Pack Sizes | 50 or 100 discs per pack |
Quality Control Data
| Control Organism | Expected Results |
|---|---|
| Escherichia coli ATCC® 25922 | MR: ≥23 mm; MR+BO: Similar |
| Klebsiella pneumoniae (KPC-positive strain) | MR: Reduced zone; MR+BO: Zone diameter increased by ≥5 mm |
| Pseudomonas aeruginosa ATCC® 27853 | MR: ≥18 mm; MR+BO: Similar |
| Staphylococcus aureus ATCC® 29213 | Not applicable (Gram-positive control) |
- Positive Controls: Confirm detection of KPC-producing strains.
- Negative Controls: Ensure no false-positive results in non-KPC producers.
Interpretation of Results
| Zone of Inhibition Comparison | Interpretation |
|---|---|
| MR zone = MR+BO zone | Absence of KPC production. |
| MR zone ≥5 mm smaller than MR+BO zone | Presence of KPC-producing bacteria. |
Comparison with Other Disc-Based Tests
| Test Disc | Recommended Use |
|---|---|
| Meropenem+Phenylboronic Acid (MR+BO) | Specific detection of KPC beta-lactamase producers. |
| Meropenem+EDTA (MR+ED) | Detection of metallo-beta-lactamase (MBL) producers. |
| Meropenem+Cloxacillin (MR+CL) | Detection of AmpC beta-lactamase producers. |
| Carbapenemase Combination Discs | General detection of carbapenemase-mediated resistance. |
Advantages
| Feature | Benefit |
|---|---|
| Dual Testing System | Differentiates KPC resistance from other carbapenemases. |
| High Sensitivity and Specificity | Reduces false-positive and false-negative results. |
| Ease of Use | Simplified setup minimizes errors and time. |
| Standards Compliance | Adheres to global antimicrobial susceptibility testing guidelines. |
Conclusion
The Liofilchem® Meropenem+Phenylboronic Acid (MR+BO) Antimicrobial Susceptibility Discs offer a reliable and precise method for detecting KPC-mediated carbapenem resistance in Gram-negative bacteria. Their high sensitivity, specificity, and adherence to international testing standards make them essential for diagnostic laboratories and resistance monitoring programs.
No resources are currently available for this product.