Liofilchem® Cefixime CFM, Antimicrobial Susceptibility Discs, 5 µg

Liofilchem® Cefixime Discs (5 µg) are high-quality antimicrobial susceptibility testing (AST) discs designed for in vitro diagnostic use using the Kirby-Bauer disk diffusion method on Mueller-Hinton agar. Each disc contains 5 micrograms of Cefixime, a third-generation oral cephalosporin antibiotic with broad-spectrum activity against Gram-negative pathogens. These discs are manufactured under strict ISO 9001 and ISO 13485 certified quality systems, ensuring accurate, consistent results in clinical, surveillance, and research microbiology laboratories.

Key Features

• Standardized dosage: Each disc contains precisely 5 µg of Cefixime, offering reliable potency.
• Broad-spectrum coverage: Particularly effective against Neisseria gonorrhoeae, Escherichia coli, Proteus mirabilis, and Haemophilus influenzae.
• Global compliance: Validated against CLSI and EUCAST interpretive criteria for susceptibility testing.
• Convenient and sterile: Supplied in ready-to-use cartridges or canisters with desiccant to maintain disc integrity.
• Color-coded disc labels: For easy visual identification and traceability.

Applications and Use Cases

1. Clinical Microbiology

Used in diagnostic laboratories to:

• Determine the susceptibility of pathogens causing:
  • Gonorrhea
  • Urinary tract infections (UTIs)
  • Respiratory tract infections
  • Typhoid fever and gastrointestinal infections
• Aid clinicians in selecting Cefixime as part of oral therapy regimens in outpatient and community health settings.

2. Public Health Surveillance

• Monitor resistance trends in Neisseria gonorrhoeae, particularly in response to global concern over declining cefixime susceptibility.
• Support national antimicrobial resistance (AMR) programs and policy formulation.

3. Research and Development

• Investigate mechanisms of resistance to third-generation cephalosporins in Gram-negative organisms.
• Evaluate potential synergy or antagonism in combination therapies involving Cefixime.
• Validate new diagnostic platforms against standard disc diffusion results.

Use Instructions

1. Prepare Mueller-Hinton agar plates (supplemented with 5% CO₂ if testing N. gonorrhoeae).
2. Standardize the bacterial inoculum to 0.5 McFarland turbidity.
3. Inoculate the plate uniformly using a sterile swab.
4. Apply the Cefixime disc (5 µg) to the agar surface using sterile forceps or a dispenser.
5. Incubate at 35 ± 2 °C for 16–20 hours (depending on organism and testing standard).
6. Measure the inhibition zone in millimeters.
7. Interpret results using the latest CLSI or EUCAST guidelines specific to the tested species.

Storage and Shelf Life

• Storage Conditions: 2–8 °C in the original packaging with desiccant.
• Shelf Life: Typically 24–36 months from the date of manufacture when stored as recommended.

Liofilchem® Cefixime CFM Discs (5 µg) provide a robust and reliable tool for testing susceptibility to an important oral third-generation cephalosporin. With proven activity against a range of Gram-negative bacteria and particular relevance in gonococcal resistance surveillance, these discs support targeted antimicrobial therapy, public health initiatives, and microbiological research. Their precision, compliance, and quality make them a trusted choice for laboratories worldwide.