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Liofilchem® Nystatin Ny Antimicrobial Susceptibility Disc, 100 IU
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Specifications:
| Application | Microbiology |
| Storage Temperature | 2-8°C |
| Product Type | Antibiotic Disc |
| Product Brand | Liofilchem |
| Product Grade | Microbiology grade |
Standardized Susceptibility Testing for Fungal Infections Using Kirby-Bauer Disc Diffusion Method
The Liofilchem® Nystatin Ny Antimicrobial Susceptibility Disc (100 IU) is a high-quality diagnostic tool used for fungal susceptibility testing in clinical and research laboratories. These pre-impregnated antibiotic discs are designed for quantitative antifungal susceptibility testing (AFST) against yeasts and fungi, particularly Candida spp. and other pathogenic fungi.
This disc is intended for use in the Kirby-Bauer disc diffusion method, which helps determine fungal resistance or susceptibility to Nystatin, a polyene antifungal agent that disrupts fungal cell membranes by binding to ergosterol. These discs comply with Clinical and Laboratory Standards Institute (CLSI) and EUCAST (European Committee on Antimicrobial Susceptibility Testing) guidelines, ensuring accurate and reproducible results in clinical microbiology laboratories.
Key Features & Benefits
✅ Standardized 100 IU Nystatin Disc – Ensures consistent and reliable antifungal susceptibility testing
✅ CLSI & EUCAST Compliant – Meets international antifungal susceptibility testing guidelines
✅ Effective Against Candida spp. & Other Yeasts – Primarily used for fungal pathogen susceptibility assessment
✅ Ready-to-Use Format – Pre-impregnated discs allow direct application onto agar plates
✅ Long Shelf Life & Stability – Manufactured for extended storage and optimal performance
✅ Ideal for Kirby-Bauer Disc Diffusion Method – Provides accurate zone diameter measurement for antifungal susceptibility testing
✅ Essential for Clinical & Research Microbiology – Aids in guiding antifungal therapy decisions
Technical Specifications
| Attribute | Details |
|---|---|
| Product Name | Liofilchem® Nystatin Ny Antimicrobial Susceptibility Disc |
| Active Ingredient | Nystatin |
| Concentration | 100 IU (International Units) |
| Disc Diameter | 6 mm |
| Testing Method | Kirby-Bauer Disc Diffusion |
| Target Organisms | Candida spp., Aspergillus spp., and other fungi |
| Storage Conditions | 2–8°C, dry environment |
| Regulatory Compliance | CLSI & EUCAST Standards |
| Format | Cartridge with multiple discs |
| Application | Antifungal susceptibility testing |
Use Cases & Applications
1. Candida Susceptibility Testing
🔬 Clinical Microbiology & Infectious Disease Labs
- Determines susceptibility of Candida spp. to Nystatin.
- Helps in antifungal therapy selection for oral, vaginal, and systemic candidiasis.
2. Aspergillus & Other Fungal Pathogen Testing
🦠 Medical Mycology & Hospital Infection Control
- Evaluates Nystatin susceptibility in filamentous fungi.
- Supports diagnosis and treatment of invasive fungal infections.
3. Antifungal Resistance Surveillance
🏥 Epidemiological & Public Health Studies
- Monitors emerging Nystatin-resistant fungal strains.
- Supports antimicrobial stewardship programs.
4. Pharmaceutical & Drug Development Research
⚗️ Antifungal Drug Susceptibility Studies
- Helps in screening for new antifungal formulations.
- Provides quality control in antifungal drug manufacturing.
How to Use the Liofilchem® Nystatin 100 IU Discs in the Kirby-Bauer Method
1️⃣ Prepare a standardized fungal inoculum (0.5 McFarland standard).
2️⃣ Evenly spread the fungal suspension onto Mueller-Hinton agar with glucose and methylene blue (MHA-GMB) or Sabouraud dextrose agar.
3️⃣ Place Nystatin Ny 100 IU discs onto the agar surface using sterile forceps or an automatic disc dispenser.
4️⃣ Incubate at 35 ± 2°C for 24–48 hours (conditions vary for different fungi).
5️⃣ Measure the zone of inhibition around the disc using a calibrated ruler or automated zone reader.
6️⃣ Compare results with CLSI or EUCAST breakpoints to determine susceptibility or resistance.
Interpretation of Results (CLSI/EUCAST Guidelines)
| Fungal Species | Susceptible (S) Zone Diameter (≥ mm) | Resistant (R) Zone Diameter (≤ mm) |
|---|---|---|
| Candida albicans | ≥ 18 mm | ≤ 14 mm |
| Candida glabrata | ≥ 16 mm | ≤ 12 mm |
| Aspergillus spp. | ≥ 20 mm | ≤ 16 mm |
(Values may be updated based on CLSI/EUCAST guidelines; always refer to the latest breakpoint tables for accurate interpretation.)
Ordering Information
| Pack Size | Catalog Number |
|---|---|
| 50 Discs per Cartridge | [Supplier SKU] |
Storage & Handling
- Store at 2–8°C in a dry, contamination-free environment.
- Do not expose discs to moisture or excessive heat to maintain potency.
- Use aseptic techniques when handling discs to prevent contamination.
- Check expiration date before use to ensure reliable results.
Regulatory & Safety Information
⚠️ For In Vitro Diagnostic (IVD) Use Only. Not for human or animal therapeutic applications.
⚠️ Handle Biosafety Level 2 (BSL-2) Pathogens with Care – Use proper biosafety precautions when testing fungal cultures.
⚠️ Follow CLSI & EUCAST Guidelines – Ensure standardized antifungal susceptibility testing methods.
Why Choose Liofilchem® Nystatin Ny Antimicrobial Susceptibility Discs (100 IU)?
🔹 CLSI & EUCAST Compliant for Reliable AST Results
🔹 Standardized 100 IU Nystatin Disc for Consistent Testing
🔹 Effective for Candida spp., Aspergillus spp., and Other Fungal Pathogens
🔹 Ideal for Hospital, Research, & Public Health Laboratories
🔹 Ready-to-Use Format for Convenient & Accurate Testing
For bulk orders, technical support, or additional information, contact LabMart Limited today. 🚀