Liofilchem® Fluconazole Flu Antimicrobial Susceptibility Disc, 100 µg

Liofilchem® Fluconazole FLU Discs (100 µg) are high-quality antifungal susceptibility testing (AFST) discs designed for in vitro use with the Kirby-Bauer disk diffusion method, specifically for testing the susceptibility of yeasts and fungi, including Candida spp. and Cryptococcus neoformans. Each disc contains 100 µg of Fluconazole, a widely used triazole antifungal agent with proven efficacy against many opportunistic and systemic fungal infections. These discs are manufactured under ISO 9001 and ISO 13485 certified quality systems, ensuring consistent performance in clinical, pharmaceutical, and research mycology applications.

Key Features

• Standardized potency: Each disc contains 100 µg of Fluconazole, suitable for zone diameter-based susceptibility testing.
• Broad antifungal activity: Active against Candida albicans, Candida glabrata, Candida parapsilosis, Cryptococcus neoformans, and other yeasts.
• CLSI and EUCAST compliant: Aligns with standardized disk diffusion protocols for fungal susceptibility testing.
• Sterile and ready-to-use: Packaged in moisture-proof cartridges or canisters with desiccant to preserve stability.
• Color-coded labeling: Facilitates quick identification in high-throughput workflows.

Applications and Use Cases

1. Clinical Mycology

Used in diagnostic laboratories to:

• Determine the susceptibility of Candida spp. and Cryptococcus spp. in cases of:
  • Oropharyngeal and esophageal candidiasis
  • Vaginal yeast infections
  • Invasive candidiasis and candidemia
  • Cryptococcal meningitis
• Support antifungal treatment decisions in immunocompromised patients, including those with HIV/AIDS, cancer, and transplant history.

2. Antifungal Resistance Monitoring

• Detect fluconazole resistance in Candida glabrata, Candida krusei, and emerging non-albicans strains.
• Monitor changes in susceptibility patterns over time and across hospital or regional populations.

3. Research and Pharmaceutical Quality Control

• Evaluate antifungal susceptibility during clinical trials or pharmaceutical QC programs.
• Study mechanisms of azole resistance in clinical or environmental isolates.
• Compare efficacy with other azoles (e.g., voriconazole, itraconazole) in antifungal R&D.

Use Instructions

1. Prepare Mueller-Hinton agar supplemented with 2% glucose and 0.5 µg/mL methylene blue (as recommended for antifungal testing).
2. Standardize the fungal inoculum to 0.5 McFarland turbidity using a spectrophotometer or turbidity standard.
3. Inoculate agar plates uniformly using a sterile swab.
4. Apply one Fluconazole disc (100 µg) using sterile forceps or an automatic disc dispenser.
5. Incubate at 35 ± 2 °C for 20–24 hours (longer for slower-growing yeasts).
6. Measure the diameter of the zone of inhibition in millimeters.
7. Interpret the results using the latest CLSI M44 or EUCAST breakpoints for relevant fungal species.

Storage and Shelf Life

• Storage Conditions: 2–8 °C in sealed packaging with desiccant.
• Shelf Life: 24–36 months from manufacturing date when stored properly.

Liofilchem® Fluconazole FLU Discs (100 µg) provide a reliable, standardized method for evaluating antifungal susceptibility in yeasts and other clinically relevant fungi. Widely used in both clinical diagnostics and research, these discs help guide effective antifungal therapy, track resistance, and support pharmaceutical development. With ISO-certified quality and alignment with international standards, they are a trusted solution for laboratories focused on fungal infections and antifungal stewardship.