Liofilchem® Doxycycline DXT, Antimicrobial Susceptibility Discs, 30 µg

Liofilchem® Doxycycline DXT Discs (30 µg) are high-quality antimicrobial susceptibility testing (AST) discs designed for use in the Kirby-Bauer disk diffusion method on Mueller-Hinton agar. Each disc is impregnated with 30 micrograms of Doxycycline, a broad-spectrum, second-generation tetracycline antibiotic used against a wide range of Gram-positive and Gram-negative bacteria, atypical organisms, and certain intracellular pathogens. Manufactured under strict ISO 9001 and ISO 13485 quality standards, these discs deliver reliable, reproducible results in clinical, veterinary, and research microbiology settings.

Key Features

• Standardized antibiotic load: Each disc contains 30 µg of doxycycline for consistent susceptibility testing.
• Broad-spectrum coverage: Effective against Staphylococcus spp., Streptococcus spp., Escherichia coli, Haemophilus influenzae, Chlamydia spp., Rickettsia spp., and others.
• Validated performance: Compliant with CLSI and EUCAST guidelines for zone interpretation.
• Sterile and stable: Supplied in sealed cartridges or canisters with desiccant to preserve potency and sterility.
• Color-coded labeling: Ensures quick and accurate identification during test setup.

Applications and Use Cases

1. Clinical Microbiology

Used in hospital and diagnostic laboratories to:

• Determine susceptibility of clinical isolates including:
  • Staphylococcus aureus (including MRSA)
  • Streptococcus pneumoniae
  • Enterobacterales (e.g., E. coli, Klebsiella pneumoniae)
  • Haemophilus influenzae, Moraxella catarrhalis
• Guide antimicrobial therapy for:
  • Respiratory tract infections
  • Skin and soft tissue infections
  • Genitourinary infections
  • Tick-borne diseases (e.g., Rickettsial infections)

2. Veterinary and Zoonotic Disease Testing

• Assess susceptibility in zoonotic pathogens affecting both animals and humans.
• Commonly used in veterinary diagnostics and One Health surveillance programs.

3. Research and Surveillance

• Monitor regional and global trends in doxycycline resistance.
• Study tetracycline resistance mechanisms (e.g., efflux pumps, ribosomal protection).
• Evaluate antimicrobial efficacy in combination studies.

Use Instructions

1. Prepare Mueller-Hinton agar plates using standard laboratory protocols.
2. Adjust the bacterial suspension to a 0.5 McFarland turbidity standard.
3. Inoculate the agar plate evenly with a sterile swab.
4. Apply one Doxycycline disc (30 µg) per plate using sterile forceps or a disc dispenser.
5. Incubate the plate at 35 ± 2 °C for 16–18 hours.
6. Measure the diameter of the inhibition zone in millimeters.
7. Interpret the results using the latest CLSI or EUCAST breakpoint tables for the relevant organism.

Storage and Shelf Life

• Storage Conditions: 2–8 °C (refrigerated) in original sealed packaging with desiccant.
• Shelf Life: 24–36 months from date of manufacture when stored properly.

Liofilchem® Doxycycline DXT Discs (30 µg) provide a dependable solution for evaluating bacterial susceptibility to a widely used broad-spectrum tetracycline. Suitable for both clinical and veterinary diagnostics, these discs play a critical role in infection management, antimicrobial resistance monitoring, and therapeutic decision-making. Backed by international standards and rigorous manufacturing quality, they are a trusted tool in modern microbiology laboratories.