Liofilchem® Kanamycin K Antimicrobial Susceptibility Discs, 30 µg

Liofilchem® Kanamycin K Antimicrobial Susceptibility Discs (30 µg) are high-quality in vitro diagnostic tools designed for antimicrobial susceptibility testing (AST) using the Kirby-Bauer disk diffusion method on Mueller-Hinton agar. Each disc is impregnated with 30 micrograms of Kanamycin, an aminoglycoside antibiotic widely used to assess resistance in Gram-negative bacilli, selected Gram-positive cocci, and Mycobacterium tuberculosis. These discs are manufactured under ISO 9001 and ISO 13485 certified systems to ensure consistent, sterile, and reliable performance in clinical, public health, and research microbiology laboratories.

Key Features

• Standardized potency: Each disc contains 30 µg of Kanamycin for accurate, reproducible AST.
• Broad-spectrum aminoglycoside: Effective against Enterobacterales, Pseudomonas spp., and Mycobacterium tuberculosis.
• AST-compliant: Suitable for CLSI and EUCAST-based disk diffusion methods.
• Sterile and stable: Packaged in sealed cartridges or canisters with desiccant to preserve potency.
• Color-coded disc labels: Easy identification during high-throughput susceptibility testing.

Applications and Use Cases

1. Clinical and Veterinary Microbiology

Used to determine susceptibility of:

• Escherichia coli, Klebsiella spp., Proteus spp., Enterobacter spp.
• Salmonella and Shigella spp.
• Pseudomonas aeruginosa and other non-fermenters
• Occasionally used in veterinary diagnostics for enteric or respiratory infections in livestock and poultry.

2. Tuberculosis and Mycobacteriology

• Included in second-line drug panels for Mycobacterium tuberculosis susceptibility testing on Lowenstein-Jensen or Middlebrook media.
• Supports resistance detection in multi-drug resistant tuberculosis (MDR-TB).

3. Antimicrobial Resistance Research

• Surveillance of aminoglycoside resistance genes and aminoglycoside-modifying enzymes (AMEs).
• Evaluate synergistic combinations with beta-lactams or colistin.
• Study efficacy of aminoglycosides against biofilm-associated infections.

Use Instructions

1. Prepare Mueller-Hinton agar (or appropriate medium for Mycobacterium if applicable).
2. Standardize inoculum to 0.5 McFarland turbidity.
3. Inoculate agar plate evenly with a sterile swab.
4. Apply the Kanamycin disc (30 µg) using sterile forceps or a disc dispenser.
5. Incubate at 35 ± 2 °C for 16–18 hours (or as per specialized protocols for mycobacteria).
6. Measure the inhibition zone in millimeters.
7. Interpret results using the latest CLSI or EUCAST breakpoint criteria.

Storage and Shelf Life

• Storage Conditions: 2–8 °C (refrigerated) in sealed packaging with desiccant.
• Shelf Life: Typically 24–36 months from manufacturing date under proper storage conditions.

Liofilchem® Kanamycin K Discs (30 µg) are essential tools for evaluating bacterial susceptibility to a key aminoglycoside antibiotic. Their broad application across clinical diagnostics, veterinary microbiology, and tuberculosis control programs makes them indispensable for resistance detection, treatment guidance, and research. Manufactured to exacting ISO standards, these discs offer precision, durability, and compliance with global AST protocols.