Liofilchem® Erythromycin E, Antimicrobial Susceptibility Discs, 15 μg

Liofilchem® Erythromycin E Discs (15 µg) are high-quality antimicrobial susceptibility testing (AST) discs designed for use in the Kirby-Bauer disk diffusion method on Mueller-Hinton agar. Each disc is impregnated with 15 micrograms of Erythromycin, a macrolide antibiotic primarily active against Gram-positive cocci, atypical respiratory pathogens, and some Gram-negative species. These discs are manufactured in accordance with ISO 9001 and ISO 13485 quality standards, ensuring reliable performance in clinical diagnostics, resistance monitoring, and microbiological research.

Key Features

• Standardized antibiotic content: Each disc contains 15 µg of Erythromycin for accurate and consistent testing.
• Broad-spectrum macrolide: Effective against Streptococcus pneumoniae, Staphylococcus aureus, Haemophilus influenzae, Mycoplasma pneumoniae, and others.
• CLSI and EUCAST compliant: Supports standardized susceptibility testing and breakpoint interpretation.
• Sterile and stable: Supplied in sealed canisters or cartridges with desiccant to ensure long-term potency.
• Color-coded labeling: Facilitates easy identification and workflow organization in laboratory settings.

Applications and Use Cases

1. Clinical Microbiology

Used in diagnostic laboratories to:

• Determine susceptibility of:
  • Staphylococcus aureus (including erythromycin-induced clindamycin resistance via D-test)
  • Streptococcus pyogenes and Streptococcus pneumoniae
  • Moraxella catarrhalis, Haemophilus influenzae
• Aid in treatment decisions for:
  • Community-acquired pneumonia (CAP)
  • Pharyngitis, otitis media, and sinusitis
  • Skin and soft tissue infections (SSTIs)

2. Resistance Surveillance and Stewardship

• Detect macrolide resistance in Streptococcus and Staphylococcus species.
• Support infection control and epidemiological programs.

3. Research and Development

• Study MLS_B (macrolide-lincosamide-streptogramin B) resistance mechanisms.
• Investigate erythromycin cross-resistance and efflux pump activity.
• Screen and compare erythromycin activity against related macrolides (e.g., azithromycin, clarithromycin).

Use Instructions

1. Prepare Mueller-Hinton agar plates following CLSI/EUCAST recommendations.
2. Standardize inoculum to 0.5 McFarland turbidity standard.
3. Inoculate agar uniformly using a sterile swab.
4. Apply one Erythromycin disc (15 µg) using sterile forceps or a disc dispenser.
5. Incubate at 35 ± 2 °C for 16–18 hours in ambient air or with 5% CO₂ depending on organism.
6. Measure the diameter of the inhibition zone in millimeters.
7. Interpret the results according to the latest CLSI or EUCAST breakpoints.

Storage and Shelf Life

• Storage Conditions: 2–8 °C (refrigerated), in original packaging with desiccant.
• Shelf Life: Typically 24–36 months from manufacturing date when unopened and properly stored.

Liofilchem® Erythromycin E Discs (15 µg) offer a reliable, standardized method for determining bacterial susceptibility to erythromycin across a wide spectrum of clinically significant pathogens. Ideal for routine diagnostics, resistance detection, and antimicrobial stewardship, these discs are essential tools in the fight against macrolide-resistant infections. With ISO-certified quality and compliance with international standards, they support accurate, reproducible results in every test.