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Pierce™ GlcNAz (N-azidoacetylglucosamine tetraacylated), 5mg
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Specifications:
| Application | Protein Biology | ||
| Storage Temperature | -20°C | ||
| Product Type | Molecular Biology Reagent | Forms | Powder |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
Azide-Labeled Sugar for Metabolic Glycoprotein Labeling & Click Chemistry
Thermo Scientific™ Pierce GlcNAz (N-Azidoacetylglucosamine-Tetraacylated) is a bioorthogonal, azide-labeled sugar designed for highly specific glycoprotein research via in vivo metabolic labeling and chemoselective ligation. This reagent is a metabolic analog of N-acetylglucosamine (GlcNAc) that allows direct tagging of glycoproteins in post-translational glycosylation pathways for detection, conjugation, and purification.
GlcNAz enables cellular incorporation of azide groups into O-linked glycosylation (O-GlcNAc-modified proteins), making it a powerful tool for glycobiology research, proteomics, and cellular pathway investigations.
Key Features & Benefits
✅ Bioorthogonal Labeling – The azide group is small, nonreactive, and absent from biological systems, preventing interference with endogenous cellular functions.
✅ Metabolic Incorporation – Mimics natural GlcNAc sugars, allowing enzymatic incorporation into glycoproteins via normal cellular glycosylation pathways.
✅ Click Chemistry Compatibility – Reacts selectively with alkyne-functionalized compounds via copper-free click chemistry or phosphine-based Staudinger ligation.
✅ Highly Specific Detection – Enables precise tracking and purification of glycosylated proteins using fluorescent dyes, biotin reagents, or affinity tags.
✅ Compatible with O-Linked Glycosylation Pathways – Specifically labels O-GlcNAc and O-GalNAc-modified proteins, distinguishing it from N-linked glycosylation analogs.
✅ Versatile Research Applications – Supports glycoproteomics, proteomics, cellular imaging, and immunoprecipitation-based assays.
✅ Stable & Easy to Use – Soluble in DMSO, stored at -20°C, and shipped at ambient temperature for convenience.
Applications
🔬 Metabolic Glycoprotein Labeling – Enables selective tagging of glycoproteins in live cells
🧪 Click Chemistry-Based Detection & Imaging – Reacts with alkyne-functionalized fluorophores for visualization
🦠 O-Linked Glycosylation Studies – Labels O-GlcNAc and O-GalNAc glycoproteins
⚗️ Proteomics & Glycoproteomics – Supports protein identification and pathway analysis
🛠 Biotinylation & Protein Purification – Enables affinity-based enrichment of glycoproteins
Technical Specifications
| Attribute | Details |
|---|---|
| Product Name | Pierce GlcNAz |
| Chemical Name | N-Azidoacetylglucosamine-Tetraacylated |
| Product Type | Azide-Labeled Sugar |
| Quantity | 5 mg |
| Chemical Reactivity | Phosphine, Alkyne |
| Reactive Moiety | Amine, Azide |
| Solubility | DMSO (Dimethyl Sulfoxide) |
| Label/Dye Type | Azide Functional Group |
| Compatible Reactions | Click Chemistry, Staudinger Ligation |
| Storage Conditions | Store at -20°C |
| Shipping Conditions | Ambient Temperature |
Mechanism of Action & Glycosylation Pathways
Glycoprotein Labeling & Detection Process:
1️⃣ GlcNAz is incorporated into cellular glycoproteins via endogenous glycosylation pathways.
2️⃣ Azide-functionalized glycoproteins are labeled with alkyne or phosphine reagents through bioorthogonal click chemistry (Cu-free).
3️⃣ Conjugated molecules (e.g., fluorescent dyes, biotin tags) allow visualization, detection, and purification.
N-Linked vs. O-Linked Glycosylation Pathways:
- O-Linked Glycosylation (O-GlcNAc & O-GalNAc) – Labeled by GlcNAz & GalNAz
- N-Linked Glycosylation – Labeled by ManNAz (N-Azidoacetylmannosamine)
Related Products
| Product Name | Function |
|---|---|
| ManNAz (N-Azidoacetylmannosamine-Tetraacylated) | Labels N-linked glycosylation in cell surface proteins |
| GalNAz (N-Azidoacetylgalactosamine-Tetraacylated) | Labels O-GalNAc-modified glycoproteins |
Recommended Experimental Workflow
- Prepare Cell Culture – Maintain cells in standard conditions with complete media.
- Metabolic Incorporation – Supplement media with GlcNAz (N-azidoacetylglucosamine) to allow cellular uptake and glycoprotein labeling.
- Cell Processing – After the desired labeling period, cells are lysed or fixed for analysis.
- Click Chemistry or Staudinger Ligation – React azide-labeled glycoproteins with alkyne-functionalized fluorophores, biotin, or affinity tags.
- Detection & Analysis – Use fluorescence microscopy, Western blotting, mass spectrometry, or flow cytometry to detect labeled glycoproteins.
Regulatory & Safety Information
⚠️ For Research Use Only. Not for diagnostic or therapeutic applications.
⚠️ Handle with Caution – Use protective gloves, eyewear, and lab coats when handling the reagent.
⚠️ Dispose of in accordance with chemical safety regulations.
Why Choose Thermo Scientific™ Pierce GlcNAz?
🔹 Highly Specific Glycoprotein Labeling – Enables selective tagging of glycosylated proteins
🔹 Compatible with Click Chemistry & Staudinger Ligation – Ensures versatile conjugation options
🔹 Stable & Easy to Use – DMSO-soluble and stored at -20°C for long-term stability
🔹 Supports Advanced Glycobiology & Proteomics Research – Ideal for cellular imaging, Western blotting, and mass spectrometry
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