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Thermo Scientific™ ActivX™ Desthiobiotin-FP Serine Hydrolase Probe is an activity-based enrichment probe designed for selective labeling and profiling of enzymatically active serine hydrolases. The probe contains a reactive fluorophosphonate (FP) group that covalently labels the active-site serine residue of functional serine hydrolases, together with a desthiobiotin tag that enables selective enrichment for downstream analysis.
This probe is useful for studying active enzyme levels, mapping active-site serine residues, profiling serine hydrolase activity, and evaluating small molecule inhibitor specificity and affinity. It supports workflows such as pull-down enrichment, Western blotting, chemiluminescent detection, and LC-MS/MS-based proteomics.
Key Features
| Feature | Details |
|---|---|
| Activity-Based Probe | Selectively labels active serine hydrolases |
| Fluorophosphonate Reactive Group | Covalently targets the active-site serine of enzymatically active hydrolases |
| Desthiobiotin Tag | Enables selective enrichment of labeled proteins or peptides |
| Functional Enzyme Profiling | Detects biologically active enzymes at the time of labeling |
| Inhibitor Screening Support | Useful for assessing inhibitor binding, specificity, and off-target effects |
| Multiple Detection Workflows | Compatible with Western blot, chemiluminescence, and mass spectrometry workflows |
| Broad Enzyme Coverage | Suitable for proteases, lipases, esterases, amidases, peptidases, and related serine hydrolases |
Applications
ActivX™ Desthiobiotin-FP Serine Hydrolase Probe is suitable for:
- Serine hydrolase activity profiling
- Detection of active serine hydrolases in cells and lysates
- Pull-down enrichment of active-site-labeled proteins
- Mapping active-site serine residues
- Profiling proteases, lipases, esterases, amidases, and peptidases
- Small molecule inhibitor screening
- Inhibitor target binding and specificity studies
- Global target and off-target identification by LC-MS/MS
- Western blot-based enzyme activity analysis
- Activity-based protein profiling workflows
Product Specifications
| Specification | Details |
|---|---|
| Product Name | ActivX™ Desthiobiotin-FP Serine Hydrolase Probe |
| Catalog Number | 88317 |
| Product Line | ActivX™ |
| Type | Fluorophosphonate, FP probe |
| Target | Serine hydrolase |
| Substrate Type | Serine hydrolase substrate |
| Formulation | Desthiobiotin-fluorophosphonate |
| Molecular Weight | 464 |
| Label / Dye | Biotin and other haptens |
| Form | Lyophilized |
| Quantity | 4.6 µg |
| Assay | Protein quantitation assay |
| Application | Pull-down |
| Detection Method | Chemiluminescence, mass spectrometry, Western blot |
| Unit Size | Each |
| Research Use | For Research Use Only; not for diagnostic procedures |
Workflow Overview
| Workflow | Description |
|---|---|
| Active-Site Labeling | FP reactive group covalently labels active-site serine residues in active serine hydrolases |
| Protein Enrichment | Desthiobiotin-labeled proteins can be enriched for SDS-PAGE and Western blot analysis |
| Peptide Enrichment | For MS workflows, labeled proteins are reduced, alkylated, digested, and enriched at the active-site peptide level |
| Inhibitor Profiling | Competitive labeling can be used to assess inhibitor target binding and off-target effects |
Ordering Information
| Catalog Number | Description | Quantity |
|---|---|---|
| 88317 | ActivX™ Desthiobiotin-FP Serine Hydrolase Probe | 4.6 µg |
Storage & Handling
Store the lyophilized probe at -80 °C, protected from light. Stock solutions prepared in DMSO can be stored at -20 °C for up to 6 months. Handle using appropriate laboratory personal protective equipment and avoid repeated freeze-thaw cycles where possible.
Research Use Statement
For Research Use Only (RUO). Not for use in diagnostic procedures. This product is subject to a limited use label license.
User Guide: ActivX Serine Hydrolase Probes
Thermo Scientific™ ActivX™ Desthiobiotin-FP Serine Hydrolase Probe is an activity-based enrichment probe designed for selective labeling and profiling of enzymatically active serine hydrolases. The probe contains a reactive fluorophosphonate (FP) group that covalently labels the active-site serine residue of functional serine hydrolases, together with a desthiobiotin tag that enables selective enrichment for downstream analysis.
This probe is useful for studying active enzyme levels, mapping active-site serine residues, profiling serine hydrolase activity, and evaluating small molecule inhibitor specificity and affinity. It supports workflows such as pull-down enrichment, Western blotting, chemiluminescent detection, and LC-MS/MS-based proteomics.
Key Features
| Feature | Details |
|---|---|
| Activity-Based Probe | Selectively labels active serine hydrolases |
| Fluorophosphonate Reactive Group | Covalently targets the active-site serine of enzymatically active hydrolases |
| Desthiobiotin Tag | Enables selective enrichment of labeled proteins or peptides |
| Functional Enzyme Profiling | Detects biologically active enzymes at the time of labeling |
| Inhibitor Screening Support | Useful for assessing inhibitor binding, specificity, and off-target effects |
| Multiple Detection Workflows | Compatible with Western blot, chemiluminescence, and mass spectrometry workflows |
| Broad Enzyme Coverage | Suitable for proteases, lipases, esterases, amidases, peptidases, and related serine hydrolases |
Applications
ActivX™ Desthiobiotin-FP Serine Hydrolase Probe is suitable for:
- Serine hydrolase activity profiling
- Detection of active serine hydrolases in cells and lysates
- Pull-down enrichment of active-site-labeled proteins
- Mapping active-site serine residues
- Profiling proteases, lipases, esterases, amidases, and peptidases
- Small molecule inhibitor screening
- Inhibitor target binding and specificity studies
- Global target and off-target identification by LC-MS/MS
- Western blot-based enzyme activity analysis
- Activity-based protein profiling workflows
Product Specifications
| Specification | Details |
|---|---|
| Product Name | ActivX™ Desthiobiotin-FP Serine Hydrolase Probe |
| Catalog Number | 88317 |
| Product Line | ActivX™ |
| Type | Fluorophosphonate, FP probe |
| Target | Serine hydrolase |
| Substrate Type | Serine hydrolase substrate |
| Formulation | Desthiobiotin-fluorophosphonate |
| Molecular Weight | 464 |
| Label / Dye | Biotin and other haptens |
| Form | Lyophilized |
| Quantity | 4.6 µg |
| Assay | Protein quantitation assay |
| Application | Pull-down |
| Detection Method | Chemiluminescence, mass spectrometry, Western blot |
| Unit Size | Each |
| Research Use | For Research Use Only; not for diagnostic procedures |
Workflow Overview
| Workflow | Description |
|---|---|
| Active-Site Labeling | FP reactive group covalently labels active-site serine residues in active serine hydrolases |
| Protein Enrichment | Desthiobiotin-labeled proteins can be enriched for SDS-PAGE and Western blot analysis |
| Peptide Enrichment | For MS workflows, labeled proteins are reduced, alkylated, digested, and enriched at the active-site peptide level |
| Inhibitor Profiling | Competitive labeling can be used to assess inhibitor target binding and off-target effects |
Ordering Information
| Catalog Number | Description | Quantity |
|---|---|---|
| 88317 | ActivX™ Desthiobiotin-FP Serine Hydrolase Probe | 4.6 µg |
Storage & Handling
Store the lyophilized probe at -80 °C, protected from light. Stock solutions prepared in DMSO can be stored at -20 °C for up to 6 months. Handle using appropriate laboratory personal protective equipment and avoid repeated freeze-thaw cycles where possible.
Research Use Statement
For Research Use Only (RUO). Not for use in diagnostic procedures. This product is subject to a limited use label license.
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