Liofilchem® Chromatic™ ESBL + AmpC Supplement, 10 x 10 mL

Liofilchem® Chromatic™ ESBL + AmpC Supplement is a selective lyophilized additive designed for use with Chromatic™ ESBL Agar Base (Ref. 610629 / 620629). The complete medium enables the isolation and detection of ESBL and AmpC β-lactamase-producing Enterobacteriaceae directly from clinical specimens.

The selective formulation promotes optimal recovery of resistant Gram-negative isolates while inhibiting carbapenem-susceptible and non-Enterobacteriaceae organisms, facilitating rapid phenotypic differentiation of β-lactamase-mediated resistance mechanisms in accordance with EUCAST and CLSI recommendations.

Key Features

• Selective supplement for detection of ESBL and AmpC-producing Enterobacteriaceae.
• Used with Chromatic™ ESBL Agar Base (Ref. 610629 / 620629).
• Differentiates ESBL and AmpC producers based on colony color reactions.
• Suppresses susceptible Enterobacteriaceae and non-target flora.
• Ready-to-reconstitute lyophilized format ensures stability and reproducibility.
• Each vial sufficient for 500 mL of medium.
• Compliant with EUCAST resistance mechanism detection guidelines.
• Ideal for clinical, hospital, and reference microbiology laboratories.

Composition

Principle of the Method

Extended-spectrum β-lactamase (ESBL) and AmpC-producing Enterobacteriaceae are among the most clinically significant antibiotic-resistant pathogens.

The Chromatic™ ESBL + AmpC Supplement contains selective components that inhibit susceptible Gram-negative bacteria while permitting the growth of resistant isolates, such as Klebsiella pneumoniae, Escherichia coli, and related species expressing ESBL or AmpC enzymes.

When combined with Chromatic™ ESBL Agar Base, the medium enables color-based differentiation of resistant strains, providing a fast and reliable phenotypic screening tool for infection control and antimicrobial resistance monitoring.

Preparation and Use

1. Aseptically reconstitute one vial of Chromatic™ ESBL + AmpC Supplement with 5 mL of sterile distilled water.
2. Mix until fully dissolved.
3. Add the entire content aseptically to 500 mL of autoclaved Chromatic™ ESBL Agar Base, cooled to 45–50°C.
4. Mix gently and pour into sterile Petri dishes.

Quality Control

Incubation: 35 ± 2°C for 18–24 hours (aerobic).

Appearance: whitish lyophilized button; clear solution after reconstitution.

Storage and Stability

• Store at 2–8°C in the original sealed container.
• Protect from humidity and direct light.
• Stable during transport up to 4 days at 18–25°C or 48 hours at 35–39°C.
• Shelf life: 4 years (as indicated on label).
• Discard if contamination or deterioration is evident.

Safety and Handling

• For in vitro diagnostic use only.
• Use by qualified microbiology professionals under aseptic conditions.
• Consult the Safety Data Sheet (SDS) before use.
• Dispose of media and cultures according to institutional and national biosafety regulations.

Ordering Information

Liofilchem® Chromatic™ ESBL + AmpC Supplement, used in conjunction with Chromatic™ ESBL Agar Base, provides a reliable, selective, and rapid screening method for detecting ESBL and AmpC-producing Enterobacteriaceae directly from clinical samples. The medium’s high selectivity and chromogenic differentiation capability make it an indispensable tool for clinical diagnostics, infection prevention, and antimicrobial resistance (AMR) surveillance in healthcare and reference laboratories.