Liofilchem® Pathogenic System AST for 20 Tests

The Liofilchem® Pathogenic System AST is a comprehensive diagnostic solution for identification and antimicrobial susceptibility testing (AST) of major pathogenic microorganisms directly from clinical specimens. This system combines rapid results, broad specimen compatibility, and ease of use, making it a valuable tool for clinical microbiology laboratories.

Key Features

1. Direct Microbial Identification:
   • Identifies a wide range of bacterial and fungal pathogens:
     • Streptococcus pyogenes (Group A)
     • Staphylococcus aureus
     • Haemophilus spp.
     • Neisseria spp.
     • Escherichia coli
     • Streptococcus agalactiae (Group B)
     • Klebsiella spp., Enterobacter spp., Serratia spp.
     • Proteus spp., Providencia spp., Pseudomonas spp.
     • Enterococcus spp. (Group D)
     • Candida spp.
2. Integrated Antimicrobial Susceptibility Testing:
   • Evaluates pathogen susceptibility to 18 antibiotics, including:
     • Aminoglycosides: Gentamicin, Netilmicin
     • Macrolides: Azithromycin, Clarithromycin
     • Beta-lactams: Amoxicillin-Clavulanic Acid, Ampicillin-Sulbactam, Piperacillin-Tazobactam
     • Cephalosporins: Ceftriaxone, Cefoperazone, Ceftazidime, Cefuroxime
     • Fluoroquinolones: Pefloxacin, Levofloxacin, Ofloxacin
     • Others: Rifampicin, Meropenem, Co-trimoxazole
3. Broad Specimen Compatibility:
   • Valid for various clinical specimens:
     • Pharyngeal swabs
     • Cutaneous (skin) swabs
     • Auricular (ear) swabs
     • Ophthalmic (eye) swabs
     • Expectorate (sputum)
     • Exudates and other sample types.
4. Rapid and Reliable:
   • Delivers results within 18–24 hours, enabling prompt clinical decision-making.
5. Ease of Use:
   • Colorimetric Results: Clear color changes simplify interpretation.
   • Confirmation Capability: Compatible with latex agglutination for result validation.

Applications

1. Clinical Diagnostics:
   • Rapidly diagnoses infections caused by bacteria and fungi affecting the skin, respiratory tract, eyes, ears, and other sites.
2. Antimicrobial Resistance Profiling:
   • Identifies resistance patterns to guide effective antibiotic therapy and promote antimicrobial stewardship.
3. Public Health and Epidemiology:
   • Monitors resistance trends and identifies emerging pathogens in healthcare settings.
4. Routine Laboratory Use:
   • Simplifies workflows in clinical microbiology laboratories by integrating identification and susceptibility testing.

Test Procedure

1. Sample Preparation:
   • Collect clinical specimens (e.g., swabs, sputum, exudates) using sterile techniques.
2. Inoculation:
   • Inoculate the panel wells with a prepared suspension of the clinical specimen.
3. Incubation:
   • Incubate the panel at 36 ± 1°C for 18–24 hours.
4. Result Interpretation:
   • Identify pathogens based on color changes in specific wells.
   • Determine susceptibility by observing growth inhibition in antibiotic-containing wells.

Benefits

Clinical Relevance

1. Diagnosis of Common Infections:
   • Detects pathogens responsible for pharyngitis, skin infections, conjunctivitis, otitis, pneumonia, and more.
2. Resistance Detection:
   • Provides detailed susceptibility profiles to inform targeted therapy and combat antimicrobial resistance.
3. Broad Pathogen Coverage:
   • Identifies key bacterial and fungal species implicated in hospital-acquired and community-acquired infections.
4. Time-Saving Diagnostics:
   • Enables laboratories to perform identification and AST in a single workflow, improving efficiency.

Comparison with Traditional Methods

Packaging Information

Regulatory Compliance

• Manufactured under ISO-certified quality systems.
• Meets guidelines from CLSI and EUCAST for identification and susceptibility testing.

Why Choose Pathogenic System AST?

• Speed: Results in under 24 hours for faster clinical decision-making.
• Accuracy: Reliable identification and AST in a single panel.
• Efficiency: Saves time, labor, and resources with integrated diagnostics.
• Simplicity: Colorimetric results for straightforward interpretation.

The Liofilchem® Pathogenic System AST is an essential tool for laboratories seeking reliable, rapid, and cost-effective diagnostics for bacterial and fungal infections.