Liofilchem® Copro System AST for 20 Tests

The Liofilchem® Copro System AST is an advanced diagnostic tool for the detection, identification, and antimicrobial susceptibility testing of intestinal pathogenic bacteria directly from fecal specimens and rectal swabs. This system streamlines laboratory workflows, delivering comprehensive results within 18–24 hours, and supports rapid clinical decision-making.

Key Features and Benefits

1. Comprehensive Diagnosis:
   • Detection and Identification:
     • Targets key intestinal pathogens, including:
       • Salmonella spp., Proteus spp., Pseudomonas spp.
       • Yersinia enterocolitica, Shigella spp., Campylobacter jejuni
       • Escherichia coli O157, Klebsiella spp., Candida spp.
     • Evaluates the intestinal flora (Escherichia coli, Enterobacter spp.).
   • Antimicrobial Susceptibility Testing:
     • Tests pathogens against commonly used antibiotics:
       • Ciprofloxacin, Ceftriaxone, Piperacillin/Tazobactam
       • Trimethoprim/Sulfamethoxazole, Rifampicin, Neomycin/Bacitracin
       • Amoxicillin/Clavulanic Acid.
2. Time-Saving and Cost-Effective:
   • Combines detection, identification, and susceptibility testing into a single system.
   • Results are available in 18–24 hours, accelerating treatment initiation.
3. User-Friendly:
   • Colorimetric Results: Clear and well-defined color changes in wells simplify interpretation.
   • Latex Agglutination Confirmation: Enables confirmation of results with rapid agglutination testing.
4. Wide Application:
   • Designed for use with fecal specimens and rectal swabs.

Clinical Applications

• Diagnosis of Intestinal Infections:
  • Detects pathogens causing foodborne and waterborne illnesses, diarrhea, dysentery, and gastroenteritis.
• Antimicrobial Resistance Profiling:
  • Provides susceptibility profiles to guide targeted antibiotic therapy.
• Flora Assessment:
  • Monitors intestinal microbiota for imbalances related to infections or medical conditions.

Test Procedure

Sample Preparation:

1. Homogenize approximately 7 grams of fecal specimen or use a rectal swab.
2. Suspend the specimen in 5 mL of physiological solution and mix thoroughly.
3. Allow the suspension to settle for 5 minutes.

Panel Inoculation:

1. Dispense 0.2 mL (4 drops) of the prepared suspension into each well of the Copro System AST panel.
2. Ensure even distribution across all wells.

Incubation:

• Incubate the panel at 36 ± 1°C for 18–24 hours.

Result Interpretation:

1. Observe color changes in the wells:
   • Positive growth is indicated by distinct color changes, simplifying pathogen identification.
2. Evaluate antimicrobial susceptibility based on growth in antibiotic-containing wells.

Advantages

Comparison with Traditional Methods

Regulatory Compliance

• Manufactured under ISO-certified quality systems.
• Aligns with CLSI and EUCAST guidelines for antimicrobial susceptibility testing.

Clinical Significance

1. Public Health Monitoring:
   • Essential for detecting outbreaks of foodborne illnesses caused by Salmonella, Shigella, or Campylobacter.
2. Antimicrobial Stewardship:
   • Provides data for selecting appropriate antibiotics, reducing misuse, and combating resistance.
3. Gastrointestinal Diagnostics:
   • Aids in diagnosing complex gastrointestinal infections and imbalances in gut flora.

The Liofilchem® Copro System AST simplifies intestinal pathogen diagnostics, combining efficiency, accuracy, and reliability into one compact system. This tool is ideal for laboratories focused on rapid, cost-effective clinical microbiology.