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Thermo Scientific™ Exonuclease VII, 1000 Units
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Specifications:
| Application | Cloning | ||
| Storage Temperature | -20°C | ||
| Product Type | Enzymes and Substrates | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
The Thermo Scientific™ Exonuclease VII is a strict single-strand–specific nuclease that uniquely exhibits both 5′→3′ and 3′→5′ exonuclease activities, making it the only bi-directional exonuclease with single-strand specificity. Unlike many nucleases, it is fully active in the presence of EDTA, demonstrating no requirement for divalent cations.
Exonuclease VII digests single-stranded DNA (ssDNA) into acid-insoluble oligonucleotides, which are further hydrolyzed into acid-soluble nucleotides. Limited digestions yield short oligonucleotides ranging from dimers to dodecamers.
The enzyme is supplied as a recombinant product from E. coli expressing the large xseA (51.8 kDa) and small xseB (8.8 kDa) subunits. With >95% purity (SDS-PAGE) and tested free of contaminating endonucleases, dsDNA exonucleases, and ribonucleases, Exonuclease VII provides high specificity and reliability for molecular biology workflows.
Key Features
- Unique Dual Directionality – The only ssDNA exonuclease with 5′→3′ and 3′→5′ activity.
- Strict ssDNA Specificity – Highly selective for single-stranded DNA; no activity on dsDNA.
- No Divalent Cation Requirement – Active in EDTA-containing reactions.
- High Purity – >95% pure; free from contaminating nucleases.
- Thermal Inactivation – Easily inactivated at 95 °C for 10 minutes.
- Reliable Activity – Produces oligonucleotides from dimers to dodecamers in controlled digestion.
Applications
- Intron mapping in genomic DNA
- Removal of vector DNA from inserts with poly(dA–dT) tails
- PCR cleanup – eliminating excess primers
- Removal of single-stranded DNA overhangs produced by restriction endonucleases
- Structural and mutational studies of DNA
Technical Specifications
| Property | Details |
|---|---|
| Product Type | Exonuclease VII |
| Catalog Number | 70082Z1000UN |
| Quantity | 1000 U |
| Concentration | 10 U/µL |
| Directionality | 5′→3′ and 3′→5′ (bi-directional) |
| Substrate Specificity | Strictly single-stranded DNA |
| Activity Definition | 1 U converts 1 nmol of nucleotide to acid-soluble form in 30 min at 37 °C |
| Optimum Temperature | 37 °C |
| Optimum pH | 8.0 |
| Inactivation | 95 °C for 10 min |
| Subunits | xseA (51.8 kDa) and xseB (8.8 kDa) |
| Source | Recombinant E. coli (xseA/xseB expression) |
| Purity | >95% (SDS-PAGE), nuclease contamination-free |
| Storage Buffer | 50 mM Tris-HCl (pH 8.0), 200 mM NaCl, 0.5 mM EDTA, 10 mM DTT, 50% glycerol |
| Storage Conditions | –20 °C |
| Shipping Conditions | Dry ice |
| Unit Size | Each |
Ordering Information
| Catalog Number | Quantity | Concentration | Unit Size |
|---|---|---|---|
| 70082Z1000UN | 1000 U | 10 U/µL | Each |
The Thermo Scientific™ Exonuclease VII is the only bi-directional ssDNA-specific exonuclease, enabling efficient removal of primers, overhangs, and vector sequences in molecular cloning and PCR workflows. Its metal ion independence, high purity, and unique substrate specificity make it indispensable for applications in DNA mapping, mutagenesis, and advanced genetic research.