Liofilchem® Malt Extract Broth

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    Specifications:
    Application Microbiology
    Storage Temperature Ambient
    Product Type Culture Medium Forms Powder
    Product Brand Liofilchem
    Product Grade Microbiology grade

    The Liofilchem® Malt Extract Broth is a liquid medium specially designed for the cultivation of yeasts and moulds. Its formulation provides essential nutrients and an acidic pH to support fungal growth while inhibiting bacterial proliferation.

    Key Features

    1. Optimized for Yeasts and Moulds:
      • Acidic pH ensures robust fungal growth and minimizes bacterial contamination.
    2. Nutrient-Rich Formula:
      • Includes malt extract, glucose, and yeast extract for enhanced organism growth.
    3. Easy Preparation:
      • Dehydrated powder allows quick and consistent preparation of the medium.
    4. Wide Application:
      • Suitable for food, pharmaceutical, and environmental fungal analyses.

    Specifications

    Formula

    ComponentAmount (g/L)
    Malt Extract6.0
    Maltose1.8
    Glucose6.0
    Yeast Extract1.2
    Final pH4.7 ± 0.2

    Physical Properties

    ParameterDetails
    Dehydrated MediumFree-flowing, homogeneous, beige
    Prepared MediumClear, light amber

    Environmental and Storage

    ConditionDetails
    Storage Temperature10-30°C (Dehydrated Medium)
    2-8°C (Prepared Medium)
    Shelf Life4 years

    Packaging Options

    ReferenceContentPackaging
    610069500 gPlastic Bottle
    620069100 gPlastic Bottle

    Applications

    1. Food Safety Testing:
      • Used to detect fungal contamination in food and beverage products.
    2. Pharmaceutical Testing:
      • Ensures safety and quality in pharmaceutical environments.
    3. Environmental Monitoring:
      • Monitors fungal growth in environmental samples.

    Usage Guidelines

    Preparation

    1. Suspend 15 g of powder in 1 liter of distilled or deionized water.
    2. Heat and stir until fully dissolved.
    3. Dispense into final containers.
    4. Sterilize at 121°C for 15 minutes.

    Technique

    1. Inoculate the medium with the test organism.
    2. Incubate at 30 ± 2°C for 18-72 hours under aerobic conditions.
    3. Observe for turbidity, indicating fungal growth.

    Microbial Performance

    MicroorganismATCC®Growth
    Aspergillus brasiliensis16404Good
    Candida albicans10231Good
    Saccharomyces cerevisiae9763Good

    Advantages

    1. Reliable Results:
      • Provides consistent fungal growth with minimized bacterial interference.
    2. Comprehensive Nutrients:
      • Supports a wide variety of fungal species.
    3. Long Shelf Life:
      • Stable for 4 years when stored correctly.
    4. Regulatory Compliance:
      • Meets industry standards for fungal detection.

    Warnings and Precautions

    1. Use only by trained personnel in a laboratory setting.
    2. Store in a dry environment to avoid contamination.
    3. Dispose of waste in accordance with local and national regulations.

    References

    1. Reddish. (1919). Abstr. Bacteriol.
    2. Thom and Church. (1926). The Aspergilli.
    3. U.S. Food and Drug Administration (2001). Bacteriological Analytical Manual, AOAC International.

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