Liofilchem® MRS Broth ISO 15214

The Liofilchem MRS Broth ISO 15214 is a high-quality, non-selective liquid medium designed for the cultivation of lactobacilli from nonclinical specimens. Its nutrient-rich composition complies with ISO/DIS 15214, making it an essential tool for the inspection of foods and the preparation of microbial inocula in microbiological assays.

Key Features:

1. Optimized for Lactobacilli Growth:
   • Supports the robust growth of mesophilic, thermophilic, and psychrophilic lactobacilli.
   • Inhibits most unwanted microorganisms due to its selective pH and specific nutrient composition.
2. Nutrient-Rich Composition:
   • Contains essential nutrients, vitamins, and growth factors derived from peptospecial, beef extract, and yeast extract.
   • Glucose serves as the primary fermentable carbohydrate.
   • Magnesium and manganese sulfates provide ions to stimulate bacterial growth.
3. Compliance with ISO Standards:
   • Fully adheres to ISO/DIS 15214 specifications for food and feed microbiological testing.
   • Ensures reproducible and reliable results in quality control workflows.
4. Flexible Incubation Protocols:
   • Suitable for different types of lactobacilli:
     • Mesophilic Lactobacilli: 30°C for 72 hours.
     • Thermophilic Lactobacilli: 48 hours at 42°C.
     • Psychrophilic Lactobacilli: 5 days at 25°C.

Typical Composition (per liter):

Preparation Instructions:

1. Suspend 54.3 g of dehydrated medium in 1 liter of distilled or deionized water.
2. Add 1 mL of Tween 80 Supplement (REF 80031).
3. Heat to boiling while stirring until the powder is completely dissolved.
4. Adjust the pH to 5.7 ± 0.1 at 25°C.
5. Distribute the solution into containers and sterilize in an autoclave at 121°C for 15 minutes.

Protocol:

1. Inoculation:
   • Inoculate the medium with the test sample or bacterial suspension.
2. Incubation:
   • Incubate at 30°C for 72 hours for mesophilic lactobacilli.
   • For specific strains, adjust incubation conditions:
     • Thermophilic: 48 hours at 42°C.
     • Psychrophilic: 5 days at 25°C.
3. Observation:
   • Monitor for turbidity, which indicates microbial growth.

Applications:

1. Food Microbiology:
   • Enumeration and cultivation of lactobacilli in fermented foods and dairy products.
2. Industrial Quality Control:
   • Suitable for monitoring probiotics and bacterial populations in food manufacturing.
3. Research and Assays:
   • Preparation of inocula for microbiological testing and genetic studies of lactic acid bacteria.

Specifications:

Quality Control:

Advantages:

1. Reliable and Consistent:
   • Optimized formulation ensures reproducible results across batches.
2. Broad Applicability:
   • Supports the growth of various lactobacilli strains under different incubation conditions.
3. ISO-Compliant:
   • Fully adheres to international standards for food microbiology testing.
4. Ease of Use:
   • Simple preparation and flexible incubation protocols make it convenient for routine laboratory use.

Storage and Handling:

• Store the dehydrated medium in a dry environment at 10–30°C.
• Prepared broth should be stored at 2–8°C away from light.
• Discard if the product shows signs of contamination or deterioration.

References:

1. Briggs, M. (1953). J. Dairy Res. 20:36.
2. De Man, J.C., Rogosa, M., and Sharpe, M.E. (1960). J. Appl. Bact. 23:130-135.
3. ISO/FDIS 15214 (1998). Microbiology of food and animal feeding stuffs—Horizontal method for enumeration of mesophilic lactic acid bacteria.

The Liofilchem MRS Broth ISO 15214 is a reliable, nutrient-rich medium designed for the cultivation and enumeration of lactobacilli in food and industrial microbiology settings. Its compliance with ISO standards ensures accuracy and reproducibility, making it a trusted choice for laboratories worldwide.