Invitrogen™ eBioscience™ CD199 (CCR9) Monoclonal Antibody (eBioCW-1.2 (CW-1.2)), eFluor™ 450, 100 µg

The Invitrogen™ CD199 (CCR9) Monoclonal Antibody (eBioCW-1.2), conjugated to eFluor™ 450, is a highly specific antibody designed for the detection of mouse CCR9 (CD199). CCR9 is a G-protein-coupled receptor (GPCR) involved in thymocyte migration and development and plays a critical role in T-cell trafficking to gut-associated lymphoid tissues. This antibody is validated for flow cytometry, offering bright fluorescence for precise analysis of CCR9 expression in immune cells.

Key Features:

1. Target and Specificity:
   • Recognizes mouse CCR9 (CD199), a receptor for thymus-expressed chemokine (TECK/CCL25).
   • High specificity ensures accurate detection in thymocytes and T cells.
2. Clone and Conjugate:
   • Clone: eBioCW-1.2 (CW-1.2), a trusted clone for reliable CCR9 detection.
   • Conjugate: eFluor™ 450, an alternative to Pacific Blue®, providing bright fluorescence with excitation at 405 nm and emission at 445 nm.
3. Validated Applications:
   • Optimized for flow cytometry, enabling robust analysis of CCR9 expression in thymocytes and immune cells.
4. Bright and Stable Fluorescence:
   • eFluor™ 450 delivers high-intensity signals with minimal background, making it ideal for multicolor flow cytometry panels.
5. Ready-to-Use:
   • Supplied at a concentration of 0.2 mg/mL, with recommended usage of 1 µg per test.

Specifications:

Applications:

1. Flow Cytometry:
   • Detect CCR9 expression in mouse thymocytes and other immune cells, including T cells.
2. Thymocyte Development Studies:
   • Analyze CCR9’s role in thymocyte migration and maturation during development.
3. Gut-Associated Immunity:
   • Study CCR9’s expression in T cells trafficking to gut-associated lymphoid tissues (GALT).
4. Chemokine Receptor Research:
   • Investigate the interaction of CCR9 with its ligand CCL25 (TECK) in immune cell trafficking.
5. Immune Cell Differentiation Studies:
   • Examine the changes in CCR9 expression during thymocyte differentiation stages.

Key Benefits:

1. Bright and Stable Fluorescence:
   • eFluor™ 450 provides superior brightness and stability for clear identification of CCR9-positive cells.
2. Reproducibility:
   • Pre-validated and optimized for flow cytometry, ensuring consistent performance.
3. Versatility:
   • Suitable for studying thymocyte development, gut-associated immunity, and chemokine receptor pathways.
4. Minimal Background:
   • High specificity of the eBioCW-1.2 clone ensures reliable results with minimal non-specific binding.
5. Multicolor Panel Compatibility:
   • eFluor™ 450’s spectral properties make it compatible with other fluorochromes in complex flow cytometry panels.

Usage Recommendations:

1. Sample Preparation:
   • Isolate mouse thymocytes or other immune cells from tissue samples.
2. Staining Protocol:
   • Use 1 µg of antibody per 100 µL of cell suspension for flow cytometry staining.
   • Incubate with cells in the dark at 4°C for 20–30 minutes.
3. Flow Cytometry:
   • Analyze samples using a flow cytometer equipped with a violet laser (405 nm).
4. Controls:
   • Include isotype controls (e.g., Mouse IgG2a kappa Isotype Control, eFluor™ 450) to assess non-specific binding.

Storage and Handling:

• Store at 4°C in the dark to protect the eFluor™ 450 fluorochrome.
• Avoid freezing, as freezing may degrade the antibody or fluorochrome.

Target Information:

• CD199 (CCR9):
  • A G-protein-coupled receptor involved in thymocyte migration and development.
  • Plays a role in T-cell trafficking to gut-associated lymphoid tissues (GALT) via interaction with its ligand CCL25 (TECK).
  • Expressed during thymocyte differentiation, peaking at the double-positive (DP) stage and downregulated in mature single-positive (SP) thymocytes.

For Research Use Only:

This product is intended for research purposes only and is not for diagnostic or therapeutic applications.