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Applied Biosystems™ QuantStudio™ 12K Flex Real-Time PCR System
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Includes 96-well interchangeable block,
Dell Laptop, QuantStudio 12K Flex Software, Power Cord Europe, Primer Express Software and Installation Kit
Specifications:
| Application | qPCR | Warranty | 1 Year |
| Storage Temperature | Room Temperature | ||
| Product Type | thermal Cycler | ||
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
The QuantStudio™ 12K Flex Real-Time PCR System is a versatile and scalable qPCR platform designed for laboratories requiring high throughput, flexibility, and robust performance. With the ability to handle multiple formats and workflows, including qPCR and digital PCR, the QuantStudio 12K Flex system is ideal for a wide range of applications in genomics, gene expression, SNP genotyping, and more.
Key Features
Flexibility & Scalability
- Interchangeable Blocks:
- Supports 96-well, Fast 96-well, 384-well, TaqMan array cards, and OpenArray plates.
- Scalable throughput, from 1 to 12,000 data points per run.
- Seamlessly switches between qPCR and digital PCR workflows.
- TaqMan Assay Compatibility:
- Optimized for a broad range of applications, including:
- Gene expression
- MicroRNA and small noncoding RNA analysis
- SNP genotyping
- Protein analysis
- High-resolution melt (HRM)
- Copy number variation (CNV)
- Pathogen detection
- Optimized for a broad range of applications, including:
Maximum Throughput
- OpenArray Format:
- Enables high-throughput genomic validation and screening with over 150,000 data points/day.
- Simplified workflow with Accufill™ System for accurate and efficient sample loading.
- Enhanced OptiFlex™ System:
- Provides superior fluorescence detection and sensitivity, ensuring consistent and reliable data analysis.
- Walk-Away Operation:
- Integrated software features, including sample tracking and a scheduling calendar, allow for unattended operation.
Software Capabilities
- QuantStudio 12K Flex Software:
- Intuitive touchscreen interface.
- Seamless data analysis across block types.
- Integration with Thermo Fisher Cloud for secure storage, sharing, and remote monitoring.
- Optional Add-Ons:
- DigitalSuite Software: Enhances sensitivity and precision for rare targets.
- ExpressionSuite Software: Analyzes over 200 384-well plates or 10 OpenArray plates for gene expression.
- 21 CFR Part 11 Compliance Module: Provides security, auditing, and electronic signatures for regulated environments.
Applications
- Gene Expression Analysis:
- Quantify mRNA levels and monitor gene regulation with precision.
- Genotyping and SNP Analysis:
- Identify genetic variations with high throughput and accuracy.
- Pathogen Detection:
- High-sensitivity detection of viral and bacterial pathogens.
- Epigenetics Research:
- Conduct methylation studies using HRM analysis.
- High-Throughput Screening:
- Screen thousands of samples daily for large-scale genomic studies.
- Digital PCR:
- Use with OpenArray plates for absolute quantification of nucleic acids.
Technical Specifications
| Feature | Details |
|---|---|
| Block Formats | Interchangeable: 96-well, Fast 96-well, 384-well, TaqMan array cards, OpenArray plates |
| Multiplexing Capability | Up to 6 targets |
| Throughput | 12,000 data points/run; >150,000 data points/day |
| Dynamic Range | 10 logs |
| Sensitivity | Detects 1.5-fold changes in target quantity |
| Touchscreen | Intuitive interface with integrated calendar |
| Connectivity | Thermo Fisher Cloud for storage and remote monitoring |
| Optional Compliance | 21 CFR Part 11 Module for secure data handling |
| Automation Integration | Compatible with Accufill™ System and robotic automation |
| Dimensions | Compact design for high-throughput laboratories |
Key Advantages
- Unmatched Throughput:
- Designed to handle high-throughput workflows, reducing experiment times from weeks to days.
- Versatility:
- Accommodates various plate formats and seamlessly switches between qPCR and digital PCR.
- Ease of Use:
- User-friendly software and touchscreen interface streamline workflows and reduce hands-on time.
- Precision and Accuracy:
- Enhanced OptiFlex technology ensures reliable, reproducible results.
- Future-Proof:
- Scalable platform allows researchers to expand applications and throughput as project needs grow.
Why Choose QuantStudio™ 12K Flex?
The QuantStudio 12K Flex is an ideal choice for researchers and labs that need:
- High-throughput capabilities for genomic studies.
- Flexibility to handle multiple workflows and applications.
- Advanced connectivity and automation for efficient operation.
- A robust system for precise and reliable qPCR and digital PCR experiments.
This system is a powerful, all-in-one solution for research labs focused on large-scale genomic validation and screening projects.
The Applied Biosystems™ QuantStudio™ series offers a range of real-time PCR (qPCR) systems tailored to various research needs. Below is a comparative overview of the QuantStudio 3, 5, 6 Flex, 7 Pro, and 12K Flex models, along with comparable instruments from other leading brands.
Thermo Fisher Scientific QuantStudio Series:
| Feature | QuantStudio 3 | QuantStudio 5 | QuantStudio 6 Flex | QuantStudio 7 Pro | QuantStudio 12K Flex |
|---|---|---|---|---|---|
| Block Formats | 96-well (0.1 mL or 0.2 mL) | 96-well (0.1 mL or 0.2 mL), 384-well | Interchangeable: 96-well (0.1 mL or 0.2 mL), 384-well | Interchangeable: 96-well (0.1 mL or 0.2 mL), 384-well, TaqMan Array Card | Interchangeable: 96-well (0.1 mL or 0.2 mL), 384-well, TaqMan Array Card, OpenArray® |
| Multiplexing Capability | Up to 4 targets | Up to 6 targets | Up to 6 targets | Up to 6 targets | Up to 6 targets |
| Dynamic Range | 9 logs | 9 logs | 9 logs | 10 logs | 9 logs |
| Sensitivity | 1.5-fold changes | 1.5-fold changes | 1.5-fold changes | 1.5-fold changes | 1.5-fold changes |
| Connectivity | Cloud-enabled | Cloud-enabled | Cloud-enabled | Cloud-enabled, facial authentication, voice commands | Cloud-enabled |
| Automation Compatibility | No | No | Yes (with upgrade) | Yes | Yes |
| Touchscreen Interface | Yes | Yes | Yes | Yes | Yes |
| High-Throughput Capability | No | No | Yes (with upgrade) | Yes | Yes (up to 110,000 data points/day) |
| Smart Features | No | No | No | Yes (facial authentication, voice commands, Smart Help) | No |
Comparable Models from Other Brands:
| Brand | Model | Block Formats | Multiplexing Capability | Dynamic Range | Notable Features |
|---|---|---|---|---|---|
| Bio-Rad | CFX96 Touch | 96-well | Up to 5 targets | 9 logs | Touchscreen interface, gradient capability, fast cycling |
| Roche | LightCycler 480 II | 96-well, 384-well | Up to 6 targets | 10 logs | High sensitivity, advanced software, versatile applications |
| Agilent | AriaMx | 96-well | Up to 6 targets | 10 logs | Modular optics, touchscreen interface, customizable filters |
| Qiagen | Rotor-Gene Q | 72-well rotor | Up to 6 targets | 10 logs | Unique rotary design for uniform thermal cycling, HRM capability |
| Eppendorf | Mastercycler RealPlex4 | 96-well | Up to 4 targets | 10 logs | Fast ramp rates, gradient function, customizable user interface |
Key Considerations:
- Throughput Needs: For high-throughput applications, the QuantStudio 12K Flex offers the highest capacity, capable of generating up to 110,000 data points per day.
- Flexibility: Models with interchangeable blocks, such as the QuantStudio 6 Flex, 7 Pro, and 12K Flex, provide versatility for various plate formats and applications.
- Advanced Features: The QuantStudio 7 Pro incorporates smart features like facial authentication and voice commands, enhancing user experience and workflow efficiency.
- Budget Constraints: The QuantStudio 3 and 5 models are more cost-effective options for laboratories with standard throughput and multiplexing requirements.
When selecting a real-time PCR system, it's essential to assess your laboratory's specific needs, including throughput, flexibility, desired features, and budget, to choose the most appropriate instrument.