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Specifications:
| Application | Capillary Electrophoresis | ||
| Storage Temperature | -15°C to -25°C | ||
| Product Type | Laboratory Chemical, Sequencing Reagents | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Applied Biosystems™ | ||
| Product Grade | Molecular Biology | Formula | CH₃NO |
Highly Deionized Formamide for Sample Denaturation in Capillary Electrophoresis Systems
Applied Biosystems™ Hi-Di™ Formamide is a high-purity, highly deionized (Hi-Di) reagent used to resuspend and denature nucleic acid samples—particularly DNA—prior to electrokinetic injection in capillary electrophoresis (CE) systems. This 5 mL configuration offers enhanced flexibility and reduced waste, particularly for labs processing a low to moderate number of samples, as it helps minimize freeze/thaw cycles compared to bulk volumes.
Hi-Di Formamide is critical in ensuring accurate fragment analysis, Sanger sequencing, and genotyping by promoting complete denaturation and preventing secondary structure formation. It is specifically validated for use with Applied Biosystems™ CE instruments such as the SeqStudio™, 3500 Series, and 3730/3730xl Genetic Analyzers.
Key Features & Benefits
-
Highly Deionized
Ultra-pure formulation free of contaminants that can interfere with capillary electrophoresis performance or signal quality. -
Consistent Sample Denaturation
Maintains single-stranded DNA structure for efficient separation and accurate sizing or sequencing. -
Ready-to-Use Format
Supplied in a 5 mL aliquot to reduce sample handling time and contamination risk. -
Minimizes Freeze/Thaw Degradation
Small volume reduces waste and preserves reagent integrity for low-throughput labs. -
Instrument-Compatible
Designed for use with all Applied Biosystems™ capillary electrophoresis instruments.
Applications
- Sanger Sequencing: Ensures single-stranded DNA conformation prior to CE injection
- Fragment Analysis: Used in STR genotyping, microsatellite analysis, and mutation detection
- Genotyping and Mutation Scanning: Promotes precise fragment separation and accurate base calling
- RNA Structure Studies: Occasionally used in RNA denaturation workflows
Specifications Table
| Attribute | Details |
|---|---|
| Product Name | Hi-Di™ Formamide |
| Catalog Number | 4401457 |
| Form | Liquid |
| Volume | 5 mL |
| Molecular Formula | CH₃NO |
| Molecular Weight | 45.04 g/mol |
| SMILES | NC=O |
| IUPAC Name | Formamide |
| InChI Key | ZHNUHDYFZUAESO-UHFFFAOYSA-N |
| Purity | Highly deionized |
| Storage Temperature | –15°C to –25°C |
| Shipping Conditions | Shipped on dry ice |
| Unit Size | Each (5 mL) |
| Intended Use | For Research Use Only |
| MDL Number | MFCD00007941 |
| Product Line | Applied Biosystems™ / Hi-Di™ |
Why Choose Hi-Di™ Formamide?
- Trusted by researchers for high-resolution fragment analysis and DNA sequencing
- Manufactured and quality-controlled under Applied Biosystems™ standards
- Reduces risk of data variability due to incomplete DNA denaturation
- Ideal for low-throughput workflows seeking maximum reagent stability
Applied Biosystems™ Hi-Di™ Formamide is a laboratory essential for researchers conducting capillary electrophoresis-based sequencing or fragment analysis. Its high-purity formulation and convenient small-volume format deliver consistent performance and minimized waste. Whether you're preparing STR genotyping samples or sequencing fragments, Hi-Di Formamide ensures the accuracy and reliability your workflows depend on.