Applied Biosystems™ TaqMan™ Ribosomal RNA Control Reagents for 1000 Rxns

Applied Biosystems™ TaqMan™ Ribosomal RNA Control Reagents are designed to detect the 18S ribosomal RNA (rRNA) gene. The TaqMan™ probe and primer sequences were designed following Rapid Assay Development Guidelines. The TaqMan™ probe and primer sequences are conserved among a diverse group of eukaryotes including human, rat, mouse, Xenopus, Saccharomyces, Giardia, and Arabidopsis. Ribosomal RNA levels provide an endogenous control for PCR quantitation studies.

• Pre-designed primers and TaqMan™ probe eliminate assay design

• Rapid Assay Development Guidelines can minimize optimization time

• Separate components provide flexibility in assay set-up

Comprehensive Guidelines Included

Applied Biosystems™ has developed a comprehensive set of guidelines to ensure success when using Applied Biosystems™ Real-Time PCR reagents and instrumentation. These guidelines are simple and easy to follow. Many traditional variables, such as magnesium chloride concentration and the thermal cycling protocol itself, have been standardized, greatly reducing assay development time.

Normalization Built-In

All Applied Biosystems™ Real-Time PCR reagent kits contain a passive internal reference to normalize non-PCR related fluorescence fluctuations. Normalizing with a passive internal reference minimizes well-to-well variability that can result from a variety of causes, such as pipetting errors and sample evaporation. The passive reference is also essential for accurate results when multiple probes (with different reporter dyes) are combined in a single tube.

Notes on Use

The TaqMan™ Ribosomal RNA Control Reagents can be used with TaqMan™ Universal PCR Master Mix or TaqMan™ PCR Core Reagents for polymerase chain reaction (PCR).

The TaqMan™ Ribosomal RNA Control Reagents can be used with the TaqMan™ One-Step RT-PCR Master Mix Reagents, TaqMan™ EZ RT-PCR Core Reagents, or the TaqMan™ Gold RT-PCR Reagents for one-step reverse transcription polymerase chain reaction (RT-PCR).

Specifications

• Control Template: Human Control RNA
• Format: Tube
• GC-Rich PCR Performance: Low
• PCR Method: 1-step RT-qPCR
• Reaction Speed: Standard
• Gene Symbol: 18S
• Internal Probe Modification: TAMRA™ Quencher (3'), VIC™ (5')
• Label or Dye: VIC
• Product Line: TaqMan™
• Product Type: Real Time PCR Reagents
• Shipping Condition: Dry Ice
• Species: S. cerevisiae, Human, Rat, Xenopus, Giardia, Mouse, Arabidopsis
• Sufficient For: 1000 Reactions
• Target: 18S rRNA
• No. of Reactions: 1000 Reactions