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Thermo Scientific™ Pierce™ BCIP Substrate Powder (5-bromo-4-chloro-3'-indolylphosphate p-toluidine), 1g
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Specifications:
| Application | Western Blot, Colorimetric Assay, ELISA-Based Assays, ELISpot Assays | ||
| Storage Temperature | -20°C | ||
| Product Type | Laboratory Chemical, Molecular Biology Reagent | Forms | Powder |
| Product Brand | Thermo Fisher Scientific™, thermo Scientific | ||
| Product Grade | Molecular Biology | Formula | C₈H₆BrClNO₄P·C₇H₉N |
The Thermo Scientific™ Pierce BCIP Substrate Powder is a high-quality 5-bromo-4-chloro-3'-indolylphosphate p-toluidine salt (BCIP) designed for use in chromogenic blotting and immunohistochemistry (IHC) applications. BCIP is commonly used in conjunction with nitro-blue tetrazolium chloride (NBT) to detect alkaline phosphatase (alk-phos) activity. When combined with NBT and reacted with alkaline phosphatase, BCIP produces an intense, insoluble black-purple formazan precipitate, which is easy to visualize and photograph without the need for special equipment.
This product is available in powder form, which allows flexibility in preparing custom solutions, or in ready-to-use solutions for convenience. BCIP, when paired with NBT, provides an excellent method for detecting alkaline phosphatase in a variety of laboratory assays, including Western blotting, immunohistochemistry, and in situ hybridization.
Key Features
- Alkaline Phosphatase Substrate: BCIP is an ideal substrate for detecting alkaline phosphatase activity in Western blotting, immunohistochemistry (IHC), and in situ hybridization assays.
- Chromogenic Detection: The reaction between BCIP and alkaline phosphatase generates a purple-black precipitate that is highly visible to the naked eye, allowing for easy documentation and analysis without the need for special imaging equipment.
- Membrane Compatibility: BCIP is compatible with common membrane types used in blotting applications, including nitrocellulose and PVDF membranes.
- Flexible Packaging Options: Available as powder for custom preparation or as preformulated ready-to-use solutions. The solution options include a choice of levamisole suppressor to minimize background interference from endogenous phosphatase activity.
Highlights
- Sensitive Detection: The BCIP and NBT combination provides highly sensitive, colorimetric detection of alkaline phosphatase activity, producing an intense blue-black precipitate that is easily detectable by eye.
- No Special Equipment Required: The reaction produces a visible precipitate, eliminating the need for specialized equipment or imaging systems.
- Ready-to-Use Solutions Available: In addition to the powder form, BCIP is available in ready-to-use solutions, which can include a levamisole suppressor to reduce the interference caused by endogenous alkaline phosphatase activity in tissue samples.
Specifications
| Property | Details |
|---|---|
| Product Type | Substrate (Powder) |
| Detection Method | Colorimetric |
| Form | Powder |
| Membrane Compatibility | Nitrocellulose, PVDF |
| Quantity | 1 g |
| Substrate | Alkaline Phosphatase |
| Unit Size | Each |
| Storage Conditions | Store at -20°C, protected from moisture. Product is shipped at ambient temperature. |
Applications
- Western Blotting: BCIP is widely used to detect alkaline phosphatase activity in Western blotting applications, providing a visual signal for the presence of specific proteins conjugated with alkaline phosphatase.
- Immunohistochemistry (IHC): Used for alkaline phosphatase detection in tissue sections and cell samples, BCIP helps researchers visualize the location and expression of target proteins.
- In Situ Hybridization: In combination with NBT, BCIP is used in in situ hybridization to detect the presence of specific nucleic acid sequences in tissue samples.
- Colony Hybridization: Used in hybridization assays to detect DNA or RNA by binding alkaline phosphatase-conjugated probes.
Preparation Notes
- Solubility: BCIP powder is soluble in water, aqueous buffers, and other solvents. The solution should be prepared at a concentration of 20 mg/mL for optimal performance.
- Absorption: BCIP itself has no significant absorption at visible wavelengths, but the reduced formazan product formed in the reaction with alkaline phosphatase absorbs strongly at 605 nm, giving a clear blue-black color.
Storage and Handling
- Storage: Store the BCIP substrate powder at -20°C to protect it from moisture and degradation. Ensure the container is tightly sealed.
- Shipping: The product is shipped at ambient temperature, and it should be stored at -20°C immediately upon receipt.
Related Products
- 1-Step™ NBT/BCIP Substrate Solution
- 1-Step™ NBT/BCIP Plus Suppressor Substrate Solution
- NBT Substrate Powder (nitro-blue tetrazolium chloride)
Important Notes
- For Life Science Research Only: This product is intended for life science research purposes only and is not for use in diagnostic procedures.
The Thermo Scientific™ BCIP Substrate Powder is a crucial reagent for alkaline phosphatase detection in colorimetric assays like Western blotting, immunohistochemistry, and in situ hybridization. It offers flexibility in experimental setup and provides reliable, visual results.