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Thermo Scientific™ Phenol saturated solution in water, stabilised with 0.08 to 0.12 % hydroxyquinoline, for molecular biology
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DNase-, RNase-, protease-, oxides-free
Specifications:
| Application | molecular biology | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Laboratory Chemical | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, thermo Scientific | ||
| Product Grade | Molecular Biology | Formula | C₆H₅OH |
Thermo Scientific™ Phenol Water-Saturated Solution is a high-purity, ready-to-use phenol/Tris-based reagent formulated specifically for molecular biology workflows. This stabilized solution is saturated with water and adjusted with Tris (hydroxymethyl)aminomethane to maintain a pH favorable for DNA extraction and purification, eliminating the need for pH titration prior to use.
Free from detectable DNase, RNase, and Protease activity, and stabilized with 0.08–0.12% hydroxyquinoline, this solution ensures sample integrity during nucleic acid isolation. It is suitable for manual or automated DNA purification workflows in research and forensic laboratories.
Key Features
- Molecular biology grade: free from DNase, RNase, and protease contamination
- Stabilized formulation: contains hydroxyquinoline (0.08–0.12%) to prevent oxidation
- Water-saturated: 25–35% water content for efficient phase separation
- pH-adjusted with Tris: no titration needed prior to use
- Color-coded: yellow to orange appearance
- Iron (Fe): ≤1 ppm
- Heavy metals (as Pb): ≤5 ppm
Applications
- DNA isolation and purification from tissues, cells, or biological fluids
- Phenol-chloroform extraction methods for molecular diagnostics
- Use in forensic DNA workflows and molecular biology sample prep
- Preparation of RNA-free DNA for downstream applications
- Phase separation of aqueous and organic fractions during nucleic acid extractions
Specifications
| Parameter | Value |
|---|---|
| Appearance (Form) | Solution |
| Appearance (Color) | Yellow to orange |
| Water Content | 25–35% |
| Stabilizer | Hydroxyquinoline (0.08–0.12%) |
| Iron (Fe) | ≤1 ppm |
| Heavy Metals (as Pb) | ≤5 ppm |
| DNase/RNase/Protease Activity | Not detected |
| Storage | Room temperature |
| Usage | For Research Use Only |
Chemical Identifiers
- CAS: 108-95-2
- IUPAC Name: Phenol
- InChI Key: ISWSIDIOOBJBQZ-UHFFFAOYSA-N
- SMILES: OC1=CC=CC=C1
Safety and Handling
Classification of the Substance or Mixture
According to CLP Regulation (EC) No 1272/2008, the product is classified as follows:
| Hazard Class | Category |
|---|---|
| Acute Oral Toxicity | Category 4 |
| Acute Dermal Toxicity | Category 3 |
| Acute Inhalation Toxicity (Vapors) | Category 3 |
| Skin Corrosion/Irritation | Category 1 |
| Serious Eye Damage/Eye Irritation | Category 1 |
| Germ Cell Mutagenicity | Category 2 |
| Specific Target Organ Toxicity – Repeated Exposure (STOT) | Category 2 |
Label Elements
| Signal Word | Danger |
|---|
Hazard Statements (H-phrases):
- H302 – Harmful if swallowed
- H311 + H331 – Toxic in contact with skin or if inhaled
- H314 – Causes severe skin burns and eye damage
- H341 – Suspected of causing genetic defects
- H373 – May cause damage to organs through prolonged or repeated exposure
Precautionary Statements (P-phrases):
- P280 – Wear protective gloves/protective clothing/eye protection/face protection
- P301 + P330 + P331 – IF SWALLOWED: Rinse mouth. Do NOT induce vomiting
- P303 + P361 + P353 – IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water or shower
- P304 + P340 – IF INHALED: Remove person to fresh air and keep comfortable for breathing
- P305 + P351 + P338 – IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
- P310 – Immediately call a POISON CENTER or doctor/physician
Thermo Scientific™ Phenol Water-Saturated Solution is a high-quality, stabilized reagent optimized for DNA extraction and nucleic acid purification. Its clean composition, pH stability, and enzyme-free profile make it an essential tool in molecular biology, genomics, and forensic science workflows.