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The Invitrogen™ Claudin 2 Monoclonal Antibody (Clone 12H12) is a highly validated antibody designed for the detection of Claudin-2, a critical tight junction protein involved in regulating paracellular permeability and epithelial barrier function. This antibody targets a C-terminal peptide of Claudin-2, enabling precise detection across a wide range of applications including Western blot, immunohistochemistry (IHC), immunocytochemistry (ICC/IF), ELISA, and immunoprecipitation.
Claudin-2 plays a key role in forming cation-selective channels (Na⁺) in epithelial tight junctions and is highly expressed in tissues such as liver and kidney, with additional roles in epithelial polarity and barrier regulation. Altered expression of Claudin-2 has been associated with cancer progression, epithelial dysfunction, and inflammatory diseases, making it an important biomarker in both physiological and pathological studies.
Key Features
- Specific for Claudin-2 tight junction protein (~22–23 kDa)
- Monoclonal antibody clone 12H12
- Reactivity: Human, Mouse, Rat, Dog
-
Broad application validation:
- Western Blot (WB): 1–3 µg/mL
- Immunohistochemistry (Paraffin): 1:10–1:100
- Immunocytochemistry (ICC/IF)
- ELISA
- Immunoprecipitation (IP)
- Verified performance in FFPE tissues and multiple cell lines
- Strong publication support (>160 references)
- Supplied as unconjugated liquid antibody
- Purified using Protein A
Applications
This antibody is ideal for:
- Western blot analysis of Claudin-2 expression
- Immunohistochemical staining of epithelial tissues
- Immunofluorescence imaging of tight junctions
- Barrier function and epithelial polarity studies
-
Cancer and epithelial disease research, including:
- Hepatocellular carcinoma
- Colon and breast cancer
- Kidney and intestinal epithelial biology
Technical Specifications
| Parameter | Specification |
|---|---|
| Product Name | Invitrogen™ Claudin 2 Monoclonal Antibody (12H12) |
| Catalog Number | 32-5600 |
| Type | Antibody |
| Class | Monoclonal |
| Clone | 12H12 |
| Host / Isotype | Mouse / IgG2b |
| Species Reactivity | Human, Mouse, Rat, Dog |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Amount | 100 µg |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Preservative | 0.1% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2533085 |
Immunogen Information
| Parameter | Details |
|---|---|
| Immunogen | Synthetic peptide corresponding to C-terminus of mouse Claudin-2 |
Target Information
Claudin-2 is a member of the claudin family of tight junction proteins that form selective barriers between epithelial cells. Unlike other claudins, Claudin-2 functions as a cation-selective channel, facilitating the paracellular transport of ions such as sodium.
Tight junctions serve both:
- A barrier function, preventing leakage of solutes between cells
- A fence function, maintaining cell polarity by restricting membrane protein diffusion
Claudin-2 is predominantly expressed in liver and kidney tissues, with additional roles in intestinal and epithelial systems. Dysregulation of Claudin-2 has been implicated in tumorigenesis, epithelial barrier dysfunction, and inflammatory diseases, making it a valuable target for both basic and translational research.
Advantages
- High specificity for tight junction protein Claudin-2
- Broad multi-application validation (WB, IHC, ICC, ELISA, IP)
- Strong literature support for research reliability
- Suitable for epithelial biology and cancer studies
- Reliable monoclonal consistency for reproducible results
The Invitrogen™ Claudin 2 Monoclonal Antibody (12H12) is a robust and versatile reagent for investigating tight junction biology, epithelial barrier function, and cancer-related changes in cell adhesion and permeability. Its extensive validation and strong performance across multiple techniques make it an essential tool for laboratories focused on cell biology, oncology, and tissue physiology.
Instructions for use
The Invitrogen™ Claudin 2 Monoclonal Antibody (Clone 12H12) is a highly validated antibody designed for the detection of Claudin-2, a critical tight junction protein involved in regulating paracellular permeability and epithelial barrier function. This antibody targets a C-terminal peptide of Claudin-2, enabling precise detection across a wide range of applications including Western blot, immunohistochemistry (IHC), immunocytochemistry (ICC/IF), ELISA, and immunoprecipitation.
Claudin-2 plays a key role in forming cation-selective channels (Na⁺) in epithelial tight junctions and is highly expressed in tissues such as liver and kidney, with additional roles in epithelial polarity and barrier regulation. Altered expression of Claudin-2 has been associated with cancer progression, epithelial dysfunction, and inflammatory diseases, making it an important biomarker in both physiological and pathological studies.
Key Features
- Specific for Claudin-2 tight junction protein (~22–23 kDa)
- Monoclonal antibody clone 12H12
- Reactivity: Human, Mouse, Rat, Dog
-
Broad application validation:
- Western Blot (WB): 1–3 µg/mL
- Immunohistochemistry (Paraffin): 1:10–1:100
- Immunocytochemistry (ICC/IF)
- ELISA
- Immunoprecipitation (IP)
- Verified performance in FFPE tissues and multiple cell lines
- Strong publication support (>160 references)
- Supplied as unconjugated liquid antibody
- Purified using Protein A
Applications
This antibody is ideal for:
- Western blot analysis of Claudin-2 expression
- Immunohistochemical staining of epithelial tissues
- Immunofluorescence imaging of tight junctions
- Barrier function and epithelial polarity studies
-
Cancer and epithelial disease research, including:
- Hepatocellular carcinoma
- Colon and breast cancer
- Kidney and intestinal epithelial biology
Technical Specifications
| Parameter | Specification |
|---|---|
| Product Name | Invitrogen™ Claudin 2 Monoclonal Antibody (12H12) |
| Catalog Number | 32-5600 |
| Type | Antibody |
| Class | Monoclonal |
| Clone | 12H12 |
| Host / Isotype | Mouse / IgG2b |
| Species Reactivity | Human, Mouse, Rat, Dog |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Amount | 100 µg |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Preservative | 0.1% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2533085 |
Immunogen Information
| Parameter | Details |
|---|---|
| Immunogen | Synthetic peptide corresponding to C-terminus of mouse Claudin-2 |
Target Information
Claudin-2 is a member of the claudin family of tight junction proteins that form selective barriers between epithelial cells. Unlike other claudins, Claudin-2 functions as a cation-selective channel, facilitating the paracellular transport of ions such as sodium.
Tight junctions serve both:
- A barrier function, preventing leakage of solutes between cells
- A fence function, maintaining cell polarity by restricting membrane protein diffusion
Claudin-2 is predominantly expressed in liver and kidney tissues, with additional roles in intestinal and epithelial systems. Dysregulation of Claudin-2 has been implicated in tumorigenesis, epithelial barrier dysfunction, and inflammatory diseases, making it a valuable target for both basic and translational research.
Advantages
- High specificity for tight junction protein Claudin-2
- Broad multi-application validation (WB, IHC, ICC, ELISA, IP)
- Strong literature support for research reliability
- Suitable for epithelial biology and cancer studies
- Reliable monoclonal consistency for reproducible results
The Invitrogen™ Claudin 2 Monoclonal Antibody (12H12) is a robust and versatile reagent for investigating tight junction biology, epithelial barrier function, and cancer-related changes in cell adhesion and permeability. Its extensive validation and strong performance across multiple techniques make it an essential tool for laboratories focused on cell biology, oncology, and tissue physiology.