Thermo Scientific™ Pierce™ Horseradish Peroxidase, Biotinylated 5mg

Thermo Scientific™ Pierce™ Horseradish Peroxidase, Biotinylated (B-HRP), is a highly purified form of the enzyme HRP conjugated to biotin. This reagent is designed for use in avidin-biotin complex (ABC) detection systems, especially for immunohistochemistry (IHC), ELISA, blotting, and other enzyme-based signal amplification methods. The covalent attachment of biotin enables HRP to be tightly and specifically bound by avidin, streptavidin, or NeutrAvidin™ conjugates for enhanced signal development in colorimetric or chemiluminescent detection protocols.

Key Features & Benefits

• Robust Signal Amplification
  – Utilizes biotin-avidin interaction to amplify HRP-based enzyme signals in IHC, ELISA, and Western blotting.
• High-Specificity Detection
  – Conjugation of biotin to HRP allows for highly specific detection using streptavidin or avidin-linked detection systems.
• Flexible Application Use
  – Ideal for ABC staining protocols, enabling strong and localized signal development in tissue samples and blots.
• Convenient Storage & Stability
  – Supplied in a lyophilized format for long-term storage and consistent activity upon reconstitution.

Applications

• Immunohistochemistry (IHC)
  – Use in ABC-based systems for high-sensitivity staining of tissue sections using biotinylated primary or secondary antibodies.
• ELISA (Enzyme-Linked Immunosorbent Assay)
  – Pair with streptavidin-coated plates for sandwich or indirect ELISAs.
• Western & Dot Blotting
  – For use in avidin- or streptavidin-based detection of biotinylated proteins or targets.
• In Situ Hybridization & Cell Staining
  – As a reporter enzyme in biotin-labeled probe systems.

Specifications Table

Thermo Scientific™ Pierce™ Biotinylated Horseradish Peroxidase is a powerful reagent designed for sensitive and specific detection of biotin-labeled molecules in a variety of biological assays. With robust enzymatic activity and efficient biotin conjugation, it serves as an essential tool in signal amplification protocols where precision and consistency are paramount.